Synthesis of Functionalized Inverse Opals Photonic Crystals and the Detection of Heparin†

doi:10.7503/cjcu20190194

Abstract

Abstract:

Heparin is the primary medicine of anticoagulant that can rapidly prevent from blood clotting, which could generate essential hemorrhage or thrombocytopenia for overusing. By functionalizing inverse opals photonic crystals(IOPCs) with polyethylene imine(PEI), the IOPCs manage to specific detect the presence of heparin via strong electrostatic interaction between them. With the concentration of heparin increasing(0—10 ^-4 mol/L), maximum peak of Bragg diffraction of functionalized IOPCs shifts about 40 nm. There’s fine Linear relation(R ²=0.99905) between logarithm of concentration of heparin and wave shifts of IOPCs, and the limit of detection is 10 ^-10 mol/L. The IOPCs obtained perform a well physical and chemical stability, which can be repeatedly used in detection of heparin with prospective practical application.

Key words: Photonic crystal, Inverse opals structure, Heparin detection, Function

CLC Number:

O648.1

TrendMD:

XU Na, LIU Zhonghu, LAN Chengwu, YIN Jianhang, CHENG Kaixuan, MENG Lei. Synthesis of Functionalized Inverse Opals Photonic Crystals and the Detection of Heparin^†[J]. Chem. J. Chinese Universities, 2019, 40(9): 1932.

Figures/Tables 8

Fig.1 SEM image(A) and diffraction spectrum(B) of PCs

Fig.2 SEM images of IOPCs(A) and functionalized IOPCs(B)

Fig.3 Diffraction spectra(A) and the corresponding digital photographs(B) of the IOPCs before(a) and after(b) functionalization with PEI

Fig.4 Diffraction spectra of the functionalized IOPCs at various concentrations of heparin solutions(A) and the diffraction peak of the functionalized IOPCs in response to heparin in aqueous solutions(B)

Fig.5 Diffraction spectra of the IOPCs without functionalized at various concentrations of heparin solutions

Fig.6 Histograms of diffraction shifts of the functiona-lized IOPCs sensing system to heparin and other important biomolecules λ and λ0 represent reflectance intensities in presence and absence of analytes. a. Heparin; b. chitosan; c. glycine; d. cysteine; e. creatinine.

Fig.7 Reversibility of response of the functionalized IOPCs to heparin(10-4 mol/L)

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