In vivo Dynamic Detection of Aloe Polysaccharides Using Carbon Fiber Microelectrodes Modified with Gold Nanoparticles †

doi:10.7503/cjcu20190422

Abstract

Abstract:

An electrochemical method for real-time dynamic detection of aloe polysaccharide in water storage tissue gel layer of aloe leaves was established. The aloe polysaccharides in aloe plants under different growth conditions were monitored in vivo by high-sensitivity carbon fiber microelectrodes modified with gold nanoparticles(AuNPs/CFME), to evaluate the real-time changes of aloe polysaccharides in the environment under stress. The experimental results show that the AuNPs/CFME has significant electrocatalytic activity to aloe polysaccharides in aloe water storage tissue. The dynamic changes of aloe polysaccharide in aloe plants under different growth environments have significant statistical differences. The modified electrode was successfully applied to the dynamic change of aloe polysaccharide in aloe plants for real-time monitoring, which made the electrochemical detection method a promising method for monitoring the photosynthesis intensity of plants.

Key words: Carbonfiber microelectrode, Gold nanoparticles, Aloe polysaccharide, Dynamic detection

CLC Number:

O657.1

TrendMD:

WANG Tingting, LI Yuan, YANG Lili, BAO Changhao, CHENG Han. In vivo Dynamic Detection of Aloe Polysaccharides Using Carbon Fiber Microelectrodes Modified with Gold Nanoparticles ^†[J]. Chem. J. Chinese Universities, 2020, 41(1): 87.

Figures/Tables 12

Fig.1 TEM image(A) and UV-Vis absorption spectrum(B) of AuNPs

Fig.2 SEM images of CFME(A) and AuNPs/CFME(B)

Fig.3 EDS image of AuNPs/CFME Inset: the red box in the SEM image of AuNPs/CFME is the detection window of EDS.

Fig.4 DPVs of aloe polysaccharide in water storage tissue gel layer of aloe leaves for AuNPs/CFME before(a) and after(b) adding 100 μL 10-2 mol/L glucose solution

Fig.5 Repetitive CV curves of aloe polysaccharide in water storage tissue gel layer of aloe leaves with scanning speed of 0.1 V/s for CFME

Fig.6 CV curves of 1 mg/mL aloe polysaccharide in PBS(pH=7.0) with scanning speed of 0.1 V/s for AuNPs/CFME

Fig.7 Repetitive CV curves of aloe polysaccharide in water storage tissue gel layer of aloe leave with scanning speed of 0.1 V/s for AuNPs/CFME

Fig.8 CV curves of aloe polysaccharide in aloe leaves water storage tissue gel layer for different electrodes

Fig.9 EIS curves of bare CFME(a), AuNPs/CFME(b) in 0.1 mol/L of KCl solution containing 5 mmol/L of [Fe(CN)6]3-/4-(A) and linear relationship between peak current and the square root of scanning rate for CFME(a) and AuNPs/CFME(b)(B) Inset of (A): Randles equivalence circuit model was used to fit the data.

Fig.10 CV curves of aloe polysaccharide in water storage tissue gel layer of aloe leaves for AuNPs/CFME with different electrodeposition time(A) and effects of electrodeposition time on the oxidation peak current of aloe polysaccharide in water storage tissue gel layer of aloe leaves by CV measurements(B) Electrodeposition time/min: a. 10; b. 20; c. 30; d. 40.

No.	I/nA		No.	I/nA
No.	Control group	Test group	No.	Control group	Test group
1	2.090	1.617	11	2.005	1.581
2	2.085	1.598	12	2.078	1.674
3	1.985	1.587	13	2.001	1.655
4	2.115	1.695	14	1.943	1.678
5	2.013	1.643	15	1.899	1.625
6	1.896	1.667	16	1.987	1.638
7	1.957	1.621	17	2.019	1.654
8	2.056	1.656	18	2.069	1.593
9	1.998	1.699	19	1.963	1.679
10	2.034	1.593	20	1.925	1.625

Fig.11 Histogram of 20 electrochemical responses of test group and control group in Table 1

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