MoS2-Gold Nanoparticles and Thionine-gold Nanoparticle Based Signal-enhanced Electrochemical Aptasensor for the Detection of 17β-Estradiol †

doi:10.7503/cjcu20190199

Abstract

Abstract:

An ultrasensitive electrochemical aptasensor for the determination of 17β-estradiol(E2) was constructed based on signal-enhanced with molybdenum disulfide nanosheet(MoS₂)-gold nanoparticles(GNPs) and thionine-gold nanoparticle(THI/GNPs) conjugates as electrochemical indicator. The aptasensor was fabricated in sequence by self-assembling of thiolated aptamer DNA probes onto the surfaces of the modified electrodes, and then hybridized with thiolated complementary strand DNA(csDNA) to form double-stranded DNA(dsDNA) duplex. MoS₂-GNPs nanoparticles were used to increase the electrode surface area, enhance the electron transfer rate and amplify the sensor signal. GNPs as a biomarker load thionine, implements the electrochemical indicator signal amplification. E2 can be detected in the concentration range from 1.0×10 ^-14 mol/L to 5.0×10 ^-12 mol/L, and down to levels as low as 4.2×10 ^-15 mol/L. This study may offer a new approach with good analytical properties and potential applications.

Key words: 17β-Estradiol, Molybdenum disulfide nanosheets, Gold nanoparticles, Thionine, Electrochemical aptasensor

CLC Number:

O657.1

TrendMD:

HE Caimei,ZHENG Jingyi,LI Xiaoxia. MoS₂-Gold Nanoparticles and Thionine-gold Nanoparticle Based Signal-enhanced Electrochemical Aptasensor for the Detection of 17β-Estradiol ^†[J]. Chem. J. Chinese Universities, 2019, 40(10): 2090.

Figures/Tables 9

Scheme 1 Schematic illustration of the aptasensor preparation and the proposed mechanism for E2 detection

Fig.1 SEM images(A, B) and EDS(C, D) spectra of the MoS2 nanosheet(A, C) and MoS2-GNPs(B, D)

Fig.2 CV(A) and EIS(B) curves of interfaces at different modified electrodes in 5.0 mmol/L [Fe(CN)6]3-/4-/10 mmol/L KCl The scan rate of CV(A) was 50 mV/s, the frequency range of EIS(B) was from 100 kHz to 1.0 Hz and the biased potential was 0.21 V. The inset shows the equivalent circuit diagram applied to fit the impedance spectroscopy. a. GCE; b. GNPs-MoS2/GCE; c. ssDNA/GNPs-MoS2/GCE; d. dsDNA/GNPs-MoS2/GCE; e. GNPs/dsDNA/GNPs-MoS2/GCE.

Fig.3 CV response of the electrochemical apta-sensor interacted with different concentrations of E2 in 0.01 mol/L Tris-HCl solution(pH=7.4) cE2/(mol·L-1): a. 0; b. 1.0×10-13; c. 1.0×10-12.

Fig.4 Optimization of immobilization time of aptamer DNA(A), GNPs(B), and the binding time of E2(C)

Fig.5 DPV profiles of the aptasensor for the detection different concentrations of E2 and plot of current response vs. E2 concentration(inset) cE2/(mol·L-1): a. 0; b. 1.0×10-14; c. 5.0×10-14; d. 1.0×10-13; e. 5.0×10-13; f. 1.0×10-12 ; g. 5.0×10-12.

Electrode	Technique	Linear range/ (mol·L^-1)	LOD/(mol·L^-1)	Sample	Ref.
Aptamer/poly(Py-co-PAA)/GCE	EIS	1.0×10^-15—1.0×10^-6	1.0×10^-15	Urine water	[14]
Aptamer/CuS/GNPs/glucose oxidase/GCE	DPV	5.0×10^-13—5.0×10^-9	6.0×10^-14	Urine	[15]
Aptamer /GNPs/WS₂/GCE	DPV	1.0×10^-11—1.0×10^-8	1.0×10^-12	Serum water	[16]
Aptamer/Au electrode	EIS	1.0×10^-11—1.0×10^-8	2.0×10^-12	Urine	[17]
Aptamer /GNPs/CoS/GCE	DPV	1.0×10^-12—1.0×10^-9	7.0×10^-13	Urine	[18]
Aptamer/dendritic Au/BDD	EIS	1.0×10^-14 —1.0×10^-9	5.0×10^-15	Water	[19]
Aptamer/gold electrode chip	SWV	1.0×10^-11—1.0×10^-9	1.0×10^-13		[20]
Aptamer/CdSe nanoparticles-modified TiO₂ nanotube arrays	PEC	5.0×10^-14—1.5×10^-11	3.3×10^-14	Water	[21]
Aptamer/ GNPs-MoS₂/GCE	DPV	1.0×10^-14—5.0×10^-12	4.2×10^-15	Water	This work

Fig.6 Histograms of response of the aptasensor interaction with E2 and E2 analogues cEZ=1.0×10-12 mol/L. a. E2; b. BPA; c. HQ; d. E3.

Sample	Added/(mol·L^-1)	Found/(mol·L^-1)	RSD(%)	Recovery(%)
1	1.00×10^-14	1.05×10^-14	3.7	105.0
2	5.00×10^-14	5.32×10^-14	4.5	106.4
3	1.00×10^-13	9.89×10^-14	4.2	98.9

References 36

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MoS₂-Gold Nanoparticles and Thionine-gold Nanoparticle Based Signal-enhanced Electrochemical Aptasensor for the Detection of 17β-Estradiol ^†

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