Fabrication of Paper-based Microfluidic Chips and the Application on the Determination of Uric Acid in Serum Based on Gold Nanoparticle-assisted Catalysis†

doi:10.7503/cjcu20160045

Abstract

Abstract:

A simple and fast method for the determiantion of uric acid(UA) in serum was developed using paper-based microfluidic chips and gold nanoparticles(AuNPs) as peroxidase mimic. The paper-based microfluidic chips were fabricated by directly drawing on filter paper with a modified gel pen filled with hydrophobic solution and then dried. When the AuNPs and the mixed solution of TMB and H₂O₂ were droped on the detection zone of paper-based microfluidic chips, the colorless TMB was oxidated to blue substance. Then the sample was added onto the blue zone, the blue-oxidated TMB was reducted to colorless. Finally the picture of detection zone could be captured by a mobile phone camera and the concentration of UA could be caclulated according to the gray value of the detection zone. Parameters influencing the UA determination were investigated, including volume of AuNPs, reaction time and temperature. Under the optimized conditions, the detection limit of UA could be reached to 4.64 mg/L, the linear range was from 10.6 mg/L to 125 mg/L and the recovery was from 94.8% to 108.5%. The method could be applied to determining uric acid in serum.

Key words: Paper-based microfluidic chip, Gold nanoparticles, Uric acid

CLC Number:

O657

TrendMD:

ZHAO Tiantian, CHEN Yuqing, ZHANG Min, WANG Yuerong, ZHANG Hongyang, HU Ping. Fabrication of Paper-based Microfluidic Chips and the Application on the Determination of Uric Acid in Serum Based on Gold Nanoparticle-assisted Catalysis^†[J]. Chem. J. Chinese Universities, 2016, 37(5): 829.

Figures/Tables 9

Fig.1 Front views(A, C) and back views(B, D) of two paper-based microfluidic chips fabricated by the modified pen with a nib width of 0.3 mm(A, B) and 0.4 mm(C, D)

Fig.2 Influence of without(A) and with(B) the addition of vacuum silicone on the resolution of paper-based microfluidic chips

Fig.3 Effects of (+)AuNPs volume on the TMB oxidation(A) and detection of UA(n=3)(B)

Fig.4 Imaging time for UA detection(n=3)a. TMB substrate; b. UA(25 mg/L); c. UA(50 mg/L); d. UA(75 mg/L).

Fig.5 Effect of temperature on the detection of UA(n=3)

Fig.6 Effects of different substances existing in serum on the UA detectiona. Blank; b.1.0 mg/L of inosine; c. 10 mg/L of creatinine; d. 7.0 mg/L of hypoxathine; e. 0.4 mg/L of xanthine; f. 1000 mg/L of glucose; g. 200 mg/L of vitamin C; h—l. 10 mg/L of NO2-, Ca2+, Na+, Cl- and Fe3+; m. 50 mg/L of UA; n. the mixture of all above(n=3).

Table 1 Recovery of UA in serum samples spiked with different concentration of UA standard solution(n=3)

c(UA)/(mg·L^-1)	Add/(mg·L^-1)	Found/(mg·L^-1)	Recovery(%)
39.6	20	58.8±4.7	96.0
39.6	40	77.5±5.1	94.8
39.6	60	104.7±7.7	108.5

Fig.7 Results of colorimetric assay of UA on paper-based microfluidic chips(A) and the calibration curve(B)#3 and #5 are samples number in Table 2.

Table 2 Results of determination of UA by paper-based microfluidic chips and HPLC(n=3)

No.	Paper-based chips	HPLC	δ(%)
#1	36.1	39.6	-8.9
#2	41.1	46.4	-11.4
#3	64.4	60.3	6.8
#4	43.4	40.3	7.7
#5	102.1	105.0	-2.8
#6	102.0	98.4	3.7
#7	51.6	57.6	-10.4
#8	96.0	100.0	-4.0

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