Influences on Adhesion and Proliferation of Endothelial Cells with AuNPs†

doi:10.7503/cjcu20150244

Abstract

Abstract:

In order to study the influence of endothelial cells behavior with different surface valences of gold nanoparticles(AuNPs), two kinds of AuNPs surfaces composed of monovalence[Au(Ⅰ)] or zero-valence gold[Au(0)], were synthesized with different reducing agent. The cell adhesion and proliferation showed that the Au(Ⅰ) on AuNPs will not affect the growth behavior of human umbilical vein endothelial cells(HUVECs) compared with Au(0). However, two kinds of AuNPs both inhibited the adhesion and proliferation of HUVECs with concentration dependence. When the concentration of AuNPs increasing from 0.01 nmol/L to 1 nmol/L, the adhesion rate of HUVECs decreased 16%, the number of cell proliferation after 7 d culture also decreased 12%. Further research showed that the inhibition of AuNPs with HUVECs was related to the immobilized state. After covalent modified onto the glass, the inhibition of AuNPs on HUVECs decreases significantly, the cell adhesion and proliferation on AuNPs modified surface were similar to the control. Vinculin and acridine orange/ethidium bromide(AO/EB) stained images also illustrated that HUVECs could spread and adhere well on the immobilized AuNPs surface, and keep a good activity on the proliferation. This study would provide a reference for the AuNPs used in biomedical applications.

Key words: Gold nanoparticles, Adhesion and proliferation endothelial cells, Surface valence, Immobilized

CLC Number:

O631

TrendMD:

WANG Bicui, WANG Wei, ZHANG Jingwei, YUAN Zhi. Influences on Adhesion and Proliferation of Endothelial Cells with AuNPs^†[J]. Chem. J. Chinese Universities, 2015, 36(8): 1619.

Figures/Tables 10

Scheme 1 Preparation of G-AuNPs

Scheme 2 Synthesis of AuNPs with different surface valences

Fig.1 Particle size(A) and zeta potential(B) of AuNPs reducing with NaBH4

Fig.2 XPS spectra of AuNPs with different surface valences(A) AuNPs(0); (B)AuNPs(Ⅰ).

Fig.3 Adhesion(A) and proliferation(B) of HUVECs in colloid AuNPs

Fig.4 XPS spectra of different glass surfaces(A) G; (B) G-NH2; (C) G-SH; (D) G-AuNPs.

Fig.5 Adhesion(A) and proliferation(B) of HUVECs on difierent surfaces

Fig.6 Fluorescent images of vinculin stained HUVECs on different surfaces(A) G; (B) G+AuNPs; (C) G-SH; (D) G-AuNPs.

Fig.7 Area of individual cell

Fig.8 AO/EB stained fluorescent images of HUVECs on different surfaces (A) G; (B) G+AuNPs; (C) G-SH; (D) G-AuNPs.

References 24

[1]	Cai W., Chen X., Small, 2007, 3(11), 1840—1854
[2]	Eustis S., El-Sayed M. A., Chem. Soc. Rev., 2006, 35(3), 209—217
[3]	Lu A. H., Salabas E. L., Schuth F., Angew. Chem. Int. Ed. Engl., 2007, 46(8), 1222—1244
[4]	Smith A. M., Nie S., Acc. Chem. Res., 2009, 43(2), 190—200
[5]	Wang L., Wang L., Xu T., Guo C., Liu C., Zhang H., Li J., Liang Z., Chem. Res. Chinese Universities, 2014, 30(6), 959—964
[6]	Fan Y., Fang Y., Chen H., Gao D., Chem. J. Chinese Universities, 2014, 35(9), 1933—1940
	(樊晔, 方云, 陈韩婷, 高迪. 高等学校化学学报, 2014, 35(9), 1933—1940)
[7]	Dreaden E. C., Mackey M. A., Huang X., Kang B., El-Sayed M. A., Chem. Soc. Rev., 2011, 40(7), 3391—3404
[8]	Scari G., Porta F., Fascio U., Avvakumova S., Dal Santo V., De Simone M., Saviano M., Leone M., Del Gatto A., Pedone C., Zaccaro L., Bioconjug. Chem., 2012, 23(3), 340—349
[9]	Wang H., Lin C. J., Lee C., Lin Y., Tseng Y., Hsieh C., Chen C., Tsai C., Hsieh C., Shen J., ACS Nano, 2011, 5(6), 4337—4344
[10]	Dreaden E. C., Alkilany A. M., Huang X., Murphy C. J., El-Sayed M. A., Chem. Soc. Rev., 2012, 41(7), 2740—2779
[11]	Chen W. H., Xu X. D., Jia H. Z., Lei Q., Luo G. F., Cheng S. X., Zhuo R. X., Zhang X. Z., Biomaterials, 2013, 34(34), 8798—8807
[12]	Kalishwaralal K., Sheikpranbabu S., BarathManiKanth S., Haribalaganesh R., Ramkumarpandian S., Gurunathan S., Angiogenesis, 2011, 14(1), 29—45
[13]	Arvizo R. R., Rana S., Miranda O. R., Bhattacharya R., Rotello V. M., Mukherjee P., Nanomedicine, 2011, 7(5), 580—587
[14]	Bhattacharya R., Mukherjee P., Adv. Drug Deliv. Rev., 2008, 60(11), 1289—1306
[15]	Mukherjee P., Bhattacharya R., Wang P., Wang L., Basu S., Nagy J. A., Atala A., Mukhopadhyay D., Soker S., Clinical Cancer Research, 2005, 11(9), 3530—3534
[16]	Bartczak D., Muskens O. L., Sanchez-Elsner T., Kanaras A. G., Millar T. M., ACS Nano, 2013, 7(6), 5628—5636
[17]	Nethi S. K., Mukherjee S., Veeriah V., Barui A. K., Chatterjee S., Patra C. R., Chem. Commun., 2014, 50(92), 14367—14370
[18]	Zhang Z., Wu Y., Langmuir, 2010, 26(12), 9214—9223
[19]	Zhang Z., Wu Y., Langmuir, 2011, 27(16), 9834—9842
[20]	Cohen-Karni T., Jeong K. J., Tsui J. H., Reznor G., Mustata M., Wanunu M., Graham A., Marks C., Bell D. C., Langer R., Kohane D. S., Nano Lett., 2012, 12(10), 5403—5406
[21]	Deng J., Yu D., Gao C., Science China Chemistry, 2013, 56(11), 1533—1541
[22]	Mao Z., Zhou X., Gao C., Biomaterials Science, 2013, 1(9), 896—911
[23]	Mao Z., Zhang Y., Li H., Tong W., Gao C., Progress in Chemistry, 2013, 25(7), 1061—1070
[24]	Zhou X., Dorn M., Vogt J., Spemann D., Yu W., Mao Z., Estrela-Lopis I., Donath E., Gao C., Nanoscale, 2014, 6(15), 8535—8542