Glucose Biosensor Based on Dy2(MoO4)3-AuNPs Composite Nanomaterial†

doi:10.7503/cjcu20160693

Abstract

Abstract:

Nanometer material of dysprosium molybdate[Dy₂(MoO₄)₃] was hydrothermally synthsized and employed for the fabrication of glucose biosensor by immobilizing glucose oxidase(GOD) on the Dy₂(MoO₄)₃-AuNPs composite nanomaterial. Dy₂(MoO₄)₃ and Dy₂(MoO₄)₃-AuNPs were characterized by transmission electron microscopy(TEM), UV-Vis spectrometry and energy-dispersive spectrometry(EDS). The electrochemical behavior of the biosensor was studied via the electrochemical impedance spectroscopy(EIS) and cyclic voltammetry(CV). The experimental results indicated that Dy₂(MoO₄)₃-AuNPs nanocomposite material presented good biocompatibility for GOD and could accelerate the electron transfer rate between the immobilized GOD and the electrode. The biosensor showed a good linear relationship in the concentration range of glucose from 0.01 mmol/L to 1.0 mmol/L with the lowest detection limit of 3.33 μmol/L(S/N=3). Additionally, the biosensor owned satisfying stability and reproducibility.

Key words: Hydrothermal method, Dysprosium molybdate, Gold nanoparticles, Glucose oxidase, Biosensor

TrendMD:

HUANG Haiping, YUE Yafeng, XU Liang, LÜ Lianlian, HU Yongmei. Glucose Biosensor Based on Dy₂(MoO₄)₃-AuNPs Composite Nanomaterial^†[J]. Chem. J. Chinese Universities, 2017, 38(4): 554.

Figures/Tables 8

Fig.1 TEM images of Dy2(MoO4)3(A) and Dy2(MoO4)3-AuNPs(B), energy spectrum of Dy2(MoO4)3(C) and UV-Vis spectra of Dy2(MoO4)3(a) and Dy2(MoO4)3-AuNPs(b)(D)

Fig.2 EIS of Dy2(MoO4)3-AuNPs/GOD/CS(a), Dy2(MoO4)3/GOD/CS(b) and GOD/CS(c) modified GCE electrodes in 0.1 mol/L KCl + 2 mmol/L [Fe(CN)6]3-/4-EIS frequency range: 0.1—1.0 × 105 Hz.

Fig.3 Cyclic voltammograms of Dy2(MoO4)3/CS(a), Dy2(MoO4)3-AuNPs/CS(b), GOD/CS(c), Dy2(MoO4)3/GOD/CS(d) and Dy2(MoO4)3-AuNPs/GOD/CS(e) modified GCE electrode in 0.1 mol/L N2-saturated PBS(pH=7.0) at the scan rate of 100 mV/s

Fig.4 Cyclic voltammograms of Dy2(MoO4)3-AuNPs/GOD/CS modified GCE at different scant rates in 0.1 mol/L N2-saturated PBS(pH=7.0)(A) and plots of cathodic peak current(Ipa) and anodic peak current(Ipc) vs. scan rate(B)From a to i, the scan rates are 10, 30, 50, 80, 100, 200, 300, 400 and 500 mV/s, respectively.

Fig.5 Cyclic voltammograms of Dy2(MoO4)3-AuNPs/GOD/CS modified GCE in PBS with different pH values(A) and plot of cathodic peak current vs. pH(B)pH: a. 5; b. 6; c. 7; d. 8.

Fig.6 Cyclic voltammograms curves of the Dy2(MoO4)3-AuNPs/GOD/CS modified GCE in the absence(a) and presence(b) of 1.0 mmol/L glucose in PBS(pH=7.0) at 100 mV/s

Fig.7 Cyclic voltammograms curves of Dy2(MoO4)3-AuNPs/GOD/CS modified GCE in 0.1 mol/L air-saturated PBS(pH=7.0) with different glucose concentrations(from a to f: 0.01, 0.05, 0.1, 0.3, 0.8, 1.0 mmol/L)(A) and calibration plot of reduction response current vs. glucose concentration(B)

Table 1 Comparison of different modified electrodes

Electrode	Linear range/(mmol·L^-1)	Detection limit/(μmol·L^-1)	Ref.
AuNPs/Al₂O₃/ GOD	1.0—42.5	10	[26]
GR/PAn/Au NPs/GOD	0.2—11.2	100	[27]
PGR-chitosan/GOD	0.14—7.0	17.5	[28]
ERGO-AuNPs/GOD	0.2—2.0	17	[29]
Dy₂(MoO₄)₃-AuNPs/GOD/CS	0.01—1.0	3.33	This work

References 29

[1]	Ali A., Hussain Z., Arain M. B., Shah N., Khan K. M., Gulab H., Zada A., Spectrochim. Acta Part B, 2016, 153, 374—378
[2]	Chen J., Ge J., Zhang L., Li Z. H., Qu L. B., Sens. Actuators B, 2016, 233, 438—444
[3]	Wang Y. Z., Zhong H., Li X. R., Liu G. Q., Yang K., Ma M., Zhang L. L., Yin J. Z., Cheng Z. P., Wang J. K., Sens. Actuators B, 2016, 230, 449—455
[4]	Si P., Huang Y. J., Wang T. H., Ma J. M., RSC Adv., 2013, 3, 3487—3502
[5]	Sen T., Mishra S., Shimpi N. G., RSC Adv., 2016, 6, 42196—42222
[6]	Kandimalla V. B., Tripathi V. S., Ju H. X., Biomaterials,2006, 27(7), 1167—1174
[7]	Xu L., Lin Y. Q., Chen X., Lu Y. L., Yang W. S., Chem. J. Chinese Universities, 2016, 37(3), 442—447
	(徐亮, 林有芹, 陈旭, 路艳罗, 杨文胜. 高等学校化学学报, 2016,37(3), 442—447)
[8]	Feng K. J., Yao Y. L., Shen G. L., Yu R. Q., Chem. J. Chinese Universities, 2012, 33(8), 1676—1680
	(封科军, 姚艳玲, 沈国励, 俞汝勤. 高等学校化学学报, 2012,33(8), 1676—1680)
[9]	Kaura B., Srivastava R., New J. Chem., 2015, 39, 6899—6906
[10]	Yu L. Y., Wei X. H., Fang C., Tu Y. F., Electrochim. Acta, 2016, 211, 27—35
[11]	Huang Y., Zhao S. L., Liu Y. M., Chen J., Chen Z. F., Shi M., Liang H., Chem. Commun., 2012, 48, 9400—9402
[12]	Ma Z. Z., Yu H. C., Wu Z. Y., Wu Y., Xiao F. B., Chin. J. Anal. Chem., 2016, 44(5), 822—826
[13]	Devasenathipathy R., Mani V., Chen S. M., Huang S. T., Huang T. T., Lind C. M., Hwa K. Y., Chen T. Y., Chen B. J., Enzyme. Microb. Tech., 2015, 78, 40—45
[14]	Sabury S., Kazemi S. H., Sharif F., Mater. Sci. Eng. C, 2015, 49, 297—304
[15]	Wang Y. Z., Shi J., Wu R. F., Li X., Zhao Y. X., Chem. Res. Chinese Universities, 2006, 22(3), 287—291
[16]	Zhao H. Y., Ji X. P., Wang B. B., Wang N., Li X. R., Ni R. X., Ren J. J., Biosens. Bioelectron., 2015, 65, 23—30
[17]	Shen C. C., Li X. Z., Rasooly A., Guo L. Y., Zhang K. N., Biosens. Bioelectron., 2016, 85, 220—225
[18]	Samdani K. J., Samdani J. S., Kim N. H., Lee J. H., Biosens. Bioelectron., 2016, 81, 445—453
[19]	Kaczmarek A.M., Hecke K. V., Deun R. V., Chem. Soc. Rev., 2015, 44,,2032—2059
[20]	Li Y., Gao Y. F., Zhou Y. Liu Y. C., Liu J. R., J. Electroanal. Chem., 2010, 642(1), 1—5
[21]	Laufer S., Strobel S., Schleid Y., Cybinska J., Mudring A., Hartenbach I., New J.Chem., 2013, 37, 1919—1926
[22]	Sen A., Pramanik P., Mater. Lett., 2001, 50(5/6), 287—294
[23]	Sun H. Y., Zhao S. G., Qu F. L., Measurement,2012, 45(5), 1111—1113
[24]	Liang R. P., Fan L. X., Huang D. M., Qiu L. D., Electroanalysis,2011, 23(3), 719—727
[25]	Sekretaryova A. N., Vokhmyanina D. V., Chulanova T. O., Karyakina E. E., Karyakin A. A., Anal. Chem., 2012, 84, 1220—1223
[26]	Zhou Y. G., Yang S., Qian Q. Y., Xia X. H., Electrochem. Commun., 2009, 11(1), 216—219
[27]	Kong F. Y., Gu S. X., Li W. W., Chen T. T., Xu Q., Wang W., Biosens. Bioelectron., 2014, 56, 77—82
[28]	Qian L., Lu L., RSC Adv., 2014, 4, 38273—38280
[29]	Wang X. L., Zhang X. L., Electrochim. Acta, 2013, 112, 774—782

Glucose Biosensor Based on Dy₂(MoO₄)₃-AuNPs Composite Nanomaterial^†

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