高等学校化学学报 ›› 2019, Vol. 40 ›› Issue (4): 639-644.doi: 10.7503/cjcu20180667

• 无机化学 • 上一篇    下一篇

脂质体@Ag/Au中空纳米壳层材料的制备及与H2O2的作用

陈艳, 董雪娇, 单桂晔()   

  1. 东北师范大学物理学院, 紫外光发射材料与技术教育部重点实验室, 长春130024
  • 收稿日期:2018-09-29 出版日期:2019-04-03 发布日期:2019-04-10
  • 作者简介:

    联系人简介: 单桂晔, 女, 博士, 教授, 主要从事纳米生物方面的研究. E-mail: shangy229@nenu.edu.cn

  • 基金资助:
    国家自然科学基金(批准号: 11740077)资助.

Preparation of Liposome@Ag/Au Nanocomposites and Their Interaction with H2O2

CHEN Yan, DONG Xuejiao, SHAN Guiye*()   

  1. School of Physics, Key Laboratory of UV-Emitting Materials and Technology of Ministry of Education, Northeast Normal University, Changchun 130024, China
  • Received:2018-09-29 Online:2019-04-03 Published:2019-04-10
  • Contact: SHAN Guiye E-mail:shangy229@nenu.edu.cn
  • Supported by:
    † Supported by the National Natural Science Foundation of China(No.11740077).

摘要:

以脂质体作为模板, 通过化学还原法制备了脂质体@Ag/Au 中空纳米材料, 并研究了其与过氧化氢(H2O2)的作用. 利用透射电子显微镜(TEM)、 紫外-可见(UV-Vis)吸收光谱和表面增强拉曼光谱(SERS)对纳米材料进行了表征. 在过氧化氢和纳米材料存在的情况下, 加入3,3 ',5,5'-四甲基联苯胺(TMB)作为显色液, 无色的TMB被氧化成蓝色的氧化TMB(oxTMB), 可以检测到反应后oxTMB在652 nm处的特征吸收强度及其拉曼光谱的变化. 实验结果表明, 当过氧化氢浓度在15100 μmol/L 范围内, oxTMB的特征吸收强度与其浓度呈线性关系, 可实现对过氧化氢的微量检测, 检测限为0.5 μmol/L.

关键词: 金, 银, 纳米材料, 类过氧化物酶, 过氧化氢

Abstract:

Liposome@Ag/Au nanocomposites were synthesized by chemical reduction using liposome as template, and their interaction with hydrogen peroxide was studied. The nanocomposites were characterized by transmission electron microscopy(TEM), UV-Vis absorption spectra and surface enhanced Raman spectra(SERS). In the presence of hydrogen peroxide and nanocomposites, 3,3',5,5'-tetramethylbenzidine(TMB) was added as the chromogenic solution, and the colorless TMB was oxidized to blue oxTMB. The characteristic absorption peak of oxTMB at 652 nm and its surface enhanced Raman spectra could be detected during the reaction. It was found that when the concentration of hydrogen peroxide was in the range of 15—100 μmol/L, the absorption intensity of oxTMB was linear with the concentration. The ultra-sensitive detection of hydrogen peroxide can be realized and the detection limit is 0.5 μmol/L.

Key words: Gold, Silver, Nanocomposites, Peroxidase-like, H2O2

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