高等学校化学学报 ›› 2011, Vol. 32 ›› Issue (8): 1709.

• 研究论文 • 上一篇    下一篇

表面等离子体共振技术用于蛋白对氨基酸手性识别的动力学研究

申刚义1,高妍1,戴东升2,崔箭1,刘裕明1, 裴凌鹏1   

  1. 1. 中央民族大学中国少数民族传统医学研究院,  中国少数民族传统医学国家民委-教育部重点实验室, 北京 100081;
    2. 亚宝药业集团股份有限公司, 太原 044602
  • 收稿日期:2011-01-21 修回日期:2011-05-19 出版日期:2011-08-10 发布日期:2011-07-19
  • 通讯作者: 申刚义;戴东升 E-mail:sgy@iccas.ac.cn;dongshengdai@126.com
  • 基金资助:

    国家自然科学基金(批准号: 81001595)、 中央高校基本科研业务费专项资金中央民族大学自主科研项目(批准号: 0910KYQN67)和新疆名医名方与特色方剂学实验室开放课题(批准号: XJDX0910-2010-01)资助.

Kinetics Study of Chiral Recognition Between Protein and Amino Acid Enantiomers by Surface Plasmon Resonance

SHEN Gang-Yi1*, GAO Yan1, DAI Dong-Sheng2*, CUI Jian1, LIU Yu-Ming1, PEI Ling-Peng1   

  1. 1. Key Laboratory of Chinese Minority Traditional Medicine, State Ethnic Affairs Commission and Ministry of Education of China, Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing 100081, China;
    2. Yabao Pharmaceutical Group Co. Ltd., Taiyuan 044602, China
  • Received:2011-01-21 Revised:2011-05-19 Online:2011-08-10 Published:2011-07-19
  • Contact: SHEN Gang-Yi;DAI Dong-Sheng E-mail:sgy@iccas.ac.cn;dongshengdai@126.com
  • Supported by:

    国家自然科学基金(批准号: 81001595)、 中央高校基本科研业务费专项资金中央民族大学自主科研项目(批准号: 0910KYQN67)和新疆名医名方与特色方剂学实验室开放课题(批准号: XJDX0910-2010-01)资助.

摘要: 利用SPR技术,以牛血清白蛋白和溶菌酶为探针构筑手性识别传感膜,开展了对L-和D-苯丙氨酸以及L-和D-色氨酸手性识别的动力学研究。实验结果表明,两种蛋白在与每种氨基酸分子的L-和D-型异构体相互作用过程都存在明显的动力学差异。动力学数据进一步显示两种蛋白与每种氨基酸L-型异构体的亲和力均大于D-型。

关键词: 表面等离子体共振, 手性识别, 氨基酸, 动力学

Abstract: Based on surface plasmon resonance technique, a new method has been developed for efficiently recognizing the chiral discrimination between the small molecule L- and D-amino acid enantiomers without any labeling. Bovine serum albumin and lysozyme were selected as chiral recognition probes, and phenylalanine and tryptophan including L-, D- four enantiomers were used as analytes. While six different concentrations of each amino acid enantiomers interacted with two proteins probes at the same time, the binding kinetics informations of them were obtained in real time. The results showed that proteins had excellent chiral recognition capability to L- and D- enantiomers. The chiral discrimination between each of amino acid enantiomers could be recognized obviously by comparison either their binding kinetics parameters or sensorgram. The results also indicated that the affinity of protein and L-amino acid was bigger than that of protein and D-amino acid.

Key words: Suface plasmon resonance(SPR), Chiral recognition, Amino acid, Kinetics

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