芹菜素的电喷雾萃取电离串联质谱

高等学校化学学报 ›› 2011, Vol. 32 ›› Issue (8): 1714.

芹菜素的电喷雾萃取电离串联质谱

丁健桦^1,2,王兴祥³,张慧¹,潘素素¹,罗明标¹,李建强¹,陈焕文^1,2

1. 东华理工大学化学生物与材料科学学院, 抚州 344000;
2. 吉林大学化学学院, 长春 130012;
3. 中国科学院南京土壤研究所, 南京 210008

收稿日期:2010-10-25 修回日期:2011-03-02 出版日期:2011-08-10 发布日期:2011-07-19
通讯作者: 陈焕文 E-mail:chw8868@gmail.com
基金资助:
科技部国际科技合作项目(批准号: 2009DFA41880)和中国科学院知识创新工程重大项目(批准号: KSCX1-YW-09-08)资助.

Extrative Electrospray Ionization Tandem Mass Spectrometry of Apigenin

DING Jian-Hua^1,2, WANG Xing-Xiang³, ZHANG Hui¹, PAN Su-Su¹, LUO Ming-Biao¹, LI Jian-Qiang¹, CHEN Huan-Wen^1,2*

1. College of Chemistry, Biology and Material Science, East China Institute of Technology, Fuzhou 344000, China;
2. College of Chemistry, Jilin University, Changchun 130012, China;
3. Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China

Received:2010-10-25 Revised:2011-03-02 Online:2011-08-10 Published:2011-07-19
Contact: CHEN Huan-Wen E-mail:chw8868@gmail.com
Supported by:
科技部国际科技合作项目(批准号: 2009DFA41880)和中国科学院知识创新工程重大项目(批准号: KSCX1-YW-09-08)资助.

摘要/Abstract

摘要： 采用实验室自制的电喷雾萃取电离源(EESI)，结合串联质谱(MSn)技术，对芹菜素这一典型的黄酮类活性化合物的质谱行为进行了研究。实验表明，在正、负离子检测模式下，该化合物均能得到较好的EESI-MS信号，且在负离子检测模式下灵敏度更高。通过对比芹菜素的EESI-MS和电喷雾电离质谱(ESI-MS)谱图发现，芹菜素在EESI-MS和ESI-MS中的裂解规律相似，但是EESI是一种比ESI更软的电离模式。根据对芹菜素EESI-MS特征碎片离子的分析，提出了芹菜素在EESI-MS中裂解的基本规律，为EESI-MS技术用于分析、鉴定复杂基质中痕量芹菜素奠定了理论和实验基础。

关键词: 芹菜素, 串联质谱, 电喷雾萃取电离质谱, 电喷雾电离质谱, 裂解规律

Abstract: The extractive electrospray ionization tandem mass spectrometry (EESI-MSn) of apigenin was studied using both positive and negative ion modes. The mechanism of the fragmentation of apigenin was discussed in detail based on the experimental collision-induced dissociation (CID) data recorded in these experiments, and was confirmed by the ESI-MS. The study is useful for the rapid determination of trace amount of apigenin in complex matrix by EESI-MS.

Key words: Apigenin, Tandem mass spectrometry, Extraction electrospray ionization mass spectrometry, Electrospray ionization mass spectrometry, Fragmentation mechanism

中图分类号:

O657

TrendMD:

丁健桦王兴祥张慧潘素素罗明标李建强陈焕文. 芹菜素的电喷雾萃取电离串联质谱. 高等学校化学学报, 2011, 32(8): 1714.

DING Jian-Hua, WANG Xing-Xiang, ZHANG Hui, PAN Su-Su, LUO Ming-Biao, LI Jian-Qiang, CHEN Huan-Wen*. Extrative Electrospray Ionization Tandem Mass Spectrometry of Apigenin. Chem. J. Chinese Universities, 2011, 32(8): 1714.

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