水溶性荧光聚合物用于测定聚赖氨酸

高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (8): 1428.

水溶性荧光聚合物用于测定聚赖氨酸

陈彦国^1,2, 何治柯¹

1. 武汉大学化学与分子科学学院, 武汉430072;
2. 湖北师范学院化学系, 黄石435002

收稿日期:2004-09-20 出版日期:2005-08-10 发布日期:2005-08-10
通讯作者: 何治柯(1963年出生),男,博士,教授,博士生导师,主要从事分子光谱研究.E-mail:zhkhe@whu.edu.cn E-mail:zhkhe@whu.edu.cn
基金资助:
国家自然科学基金(批准号:20275028);国家“八六三”计划项目(批准号:2002AA2Z2004)资助.

Determination of Poly-L-lysine with a Water-soluble Fluorescence Polymer

CHEN Yan-Guo^1,2, HE Zhi-Ke¹

1. College of Chemistry & Molecular Sciences, Wuhan University, Wuhan 430072, China;
2. Department of Chemistry, Hubei Normal University, Huangshi 435002, China

Received:2004-09-20 Online:2005-08-10 Published:2005-08-10

摘要/Abstract

摘要： 合成了一种水溶性荧光聚合物聚[5-甲氧基-2-(3-磺酰化丙氧基)-1,4-苯撑乙烯](MPS-PPV),对该聚合物进行了元素分析、红外光谱及透射电子显微镜表征.实验结果表明,聚赖氨酸(PLL)对该聚合物荧光具有明显的猝灭作用,据此建立了一种快速灵敏测定聚赖氨酸的新方法,检出限为5.0×10^-10mol/L.探讨了聚赖氨酸猝灭MPS-PPV荧光的机理.

关键词: 荧光, 聚合物, 聚[5-甲氧基-2-(3-磺酰化丙氧基)-1, 4-苯撑乙烯], 聚赖氨酸

Abstract: A water-soluble fluorescence polymer poly[5-methoxy-2-(3-sulfopropoxy)-1,4-phenylene-vinylene](MPS-PPV) was synthesized and characterized by elemental analysis, infrared absorption spectrun and the transmission electron microscope micrograph. The fluorescence of MPS-PPV could be quenched by poly-L-lysine(PLL), a new food preservative. So a rapid and sensitive method for the determination of PLL was set up, the linear range and detection limit were 3.6×10^-92.5×10^-8 mol/L and 5.0×10^-10 mol/L respectively. The fluorescence quenching mechanism of PLL to MPS-PPV was also discussed, which was caused by the strong electrostatic interaction between cationic PLL and anion conjugated polymer MPS-PPV.

Key words: Fluorescence, Polymer, Poly[5-methoxy-2-(3-sulfopropoxy)-1,4-phenylenevinylene], Poly-L-lysine

中图分类号:

O625

TrendMD:

陈彦国, 何治柯. 水溶性荧光聚合物用于测定聚赖氨酸. 高等学校化学学报, 2005, 26(8): 1428.

CHEN Yan-Guo, HE Zhi-Ke . Determination of Poly-L-lysine with a Water-soluble Fluorescence Polymer. Chem. J. Chinese Universities, 2005, 26(8): 1428.

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