高等学校化学学报

高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (8): 1424.

• 研究论文 • 上一篇    下一篇

葡萄酒中7种酚酸的色谱电化学分析

曹炜1,2, 索志荣1, 宋纪蓉2, 郑建斌1   

  1. 1. 西北大学分析科学研究所, 陕西省电分析化学重点实验室;
    2. 西北大学化学工程学院, 西安710069
  • 收稿日期:2004-09-28 出版日期:2005-08-10 发布日期:2005-08-10
  • 通讯作者: 郑建斌(1958年生),男,博士,教授,博士生导师,从事电分析及天然产物化学研究.E-mail:zhengjb@nwu.edu.cn E-mail:zhengjb@nwu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:20275030);陕西省自然科学基金(批准号:2004B20);陕西省教育厅专项研究基金项目(批准号:03JK111);西北大学基金(批准号:03NW29)资助.

Analyses of Seven Kinds of Phenolic Acids in Wine by HPLC with Electrochemical Detection

CAO Wei1,2, SUO Zhi-Rong1, SONG Ji-Rong2, ZHENG Jian-Bin1   

  1. 1. Institute of Analytical Science, Shaanxi Provincial Key Lab of Electroanalytical Chemistry;
    2. College of Chemical Engineering, Northwest University, Xi'an 710069, China
  • Received:2004-09-28 Online:2005-08-10 Published:2005-08-10

PDF (PC)

被引次数

55

摘要： 建立了同时测定葡萄酒中没食子酸、原儿茶酸、丁香酸、p-香豆酸、咖啡酸、绿原酸和阿魏酸等7种生物活性酚酸的反相高效液相色谱电化学分析新方法,并测定了5种国产不同品牌的葡萄酒.采用HypersilODS色谱柱(250mm×4.0mm,5.0μm),流动相为甲醇-4%醋酸,梯度洗脱,流速为0.8mL/min,工作电压为0.7V,柱温为30℃.实验结果表明,电化学法的检出限比紫外法的检出限低4～600倍.

关键词: 高效液相色谱法, 电化学检测, 葡萄酒, 酚酸

Abstract: A rapid, sensitive and accurate reverse phase HPLC-ECD method for simultaneous determination of seven kinds of bioactive phenolic acids(gallic acid, protocatechuic acid, syringic acid, p-coumaric acid, caffeic acid, chlorogenic acid, ferulic acid) in five different types of wine was developed. The separation was performed on Hypersil ODS column(250 mm×4.0 mm, 5.0 μm) by gradient elution. The mobile phase consisted of CH-3OH-4%HAc. The flow rate was 0.8 mL/min. The working potential was 0.7 V and column temperature was 30 ℃. The experimental results showed that the seven kinds of phenolic acids were separated satisfactorily. The detection limit of phenolic acids with ECD was 4-600 times greater than those obtained with UV detection.

Key words: HPLC, Electrochemical detection, Wine, Phenolic acid

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