高等学校化学学报

高等学校化学学报 ›› 2003, Vol. 24 ›› Issue (8): 1495.

• 论文 • 上一篇    下一篇

合成分枝型聚乙二醇的简便新方法

何明磊, 苏志国   

  1. 中国科学院过程工程研究所生化工程国家重点实验室, 北京 100080
  • 收稿日期:2002-09-30 出版日期:2003-08-24 发布日期:2003-08-24
  • 通讯作者: 苏志国(1954年出生),男,博士,研究员,主要从事生化工程研究.E-mail:Aliha1965@163.com E-mail:Aliha1965@163.com
  • 基金资助:

    国家自然科学基金(批准号:20136020)资助

A New Facile Synthesis Method of Branched PEG

HE Ming-Lei, SU Zhi-Guo    

  1. National Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China
  • Received:2002-09-30 Online:2003-08-24 Published:2003-08-24

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被引次数

29

摘要： 以赖氨酸和mPEG5000为起始物,利用多肽合成中常用的保护、缩合和脱保护等方法合成了在生物医学领域中具有重要应用价值的分枝型聚乙二醇.用该方法形成的分枝型PEG在有机相中以缩合反应的方式一步完成,反应条件温和,且有较高的产率(61%).各步产物的表征都与其结构一致.最终产物分枝型PEG的1HNMR的表征结果与其结构吻合.

关键词: 新方法, 合成, 分枝型聚乙二醇

Abstract: The modification of bioactive proteins (peptides, enzymes) by using polyethylene glycol(PEG) has still been a hot filed of research.It has been reported that branched PEG is distinctly superior to linear PEG in the modification of some enzymes.However the existing synthetic methods of branched PEG make it very expensive to be used in research and practice.In this paper, a kind of branched PEG was synthesized via conventional methods used in the synthesis of polypeptide(protection, condensation, and deprotection) using lysine and m PEG5000 as starting materials.In this method, the most important reaction of forming branched PEG was fulfilled in one step by condensation reaction in organic phase.It makes this method remarkably different from other methods.The reaction condition is mild and the yield of the product(61%) is higher.The characterization of products in different steps is consistent with their structures.The 1HNMR spectrum of our end product branched PEG tallies with its structure.

Key words: New method, Synthesis, Branched PEG

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