高等学校化学学报

高等学校化学学报 ›› 1998, Vol. 19 ›› Issue (4): 543.

• 论文 • 上一篇    下一篇

二价金属离子影响乳酸脱氢酶活性的单扫伏安研究

汪勋清, 高小霞   

  1. 北京大学化学系, 北京, 100871
  • 收稿日期:1997-03-14 出版日期:1998-04-24 发布日期:1998-04-24
  • 通讯作者: 高小霞
  • 作者简介:汪勋清,男,31岁,博士研究生.
  • 基金资助:

    国家自然科学基金

Studies on the Effects of Five Kinds of Divalent Metal Ions on LDH Activity by Single Sweep Voltammetry

WANG Xun-Qing, GAO Xiao-Xia    

  1. Department of Chemistry, Peking University, Beijing, 100871
  • Received:1997-03-14 Online:1998-04-24 Published:1998-04-24

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被引次数

6

摘要： 探讨了5种二价金属离子(Zn2+,Cu2+,Mn2+,Ca2+,Mg2+)对乳酸脱氖酶(LDH)在辅酶NADH存在下转化丙酮酸为乳酸催化体系的影响,结果表明,Zn2+、Ca2+对LDH活性无影响,Mn2+有抑制作用,而Cu2+、Mg2+在低浓度时有微弱的促进作用.从Zn2+与辅酶、酶的三元配合物的模型可以看出,LDH是一非金属酶,Zn2+不起催化作用.对Cu2+、Mg2+、Ca2+、Mn2+的不同影响作了初步解释.

关键词: 乳酸脱氢酶, 二价金属离子, 催化, 单扫伏安法

Abstract: In this paper the effects of five kinds of divalent metal ions on LDH activity were studied. The results showed that Zn 2+ and Ca 2+ do not affect LDH activity while Mn 2+ exerted inhibitory effect. At low concentrations, Cu 2+ and Mg 2+ slightly stimulate LDH activity. From the binding model of ternary complex, it could be concluded that LDH is a non-metallic enzyme and Zn 2+ did not play a catalytic role in LDH. The different effects of Cu 2+, Mg 2+, Mn 2+ and Ca 2+ ions were also discussed.

Key words: LDH, Divalent metal ions, Catalysis, Single sweep voltammetry

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