高等学校化学学报 ›› 1993, Vol. 14 ›› Issue (1): 144.

• 研究简报 • 上一篇    

胰凝乳蛋白酶显色底物的合成及动力学

张若蘅, 葛雪, 徐小杰, 唐有祺   

  1. 北京大学化学系, 北京 100871
  • 收稿日期:1992-01-11 修回日期:1992-07-18 出版日期:1993-01-24 发布日期:1993-01-24
  • 通讯作者: 张若蘅
  • 基金资助:

    国家自然科学基金;青年基金

Synthesis and Kinetics of Chromogenic Substrates for Chymotrypsin

ZHANG Ruo-Heng, GE Xue, XU Xiao-Jie, TANG You-qi   

  1. Deportmet of Chemistry, Peking University, Beijing, 100871
  • Received:1992-01-11 Revised:1992-07-18 Online:1993-01-24 Published:1993-01-24

关键词: 显色底物, 合成, 动力学, 胰凝乳蛋白酶

Abstract: Five chromogenic substrates for chymotrypsin, P3-P2-Pl-p-NA (P1,P2,P3 = Phe, Leu, Ala, Pro, Asn) have been synthesized.The influence of amino acid on the specificity was given by kinetic analysis of the hydrolysis of the peptide by bovine chymotrypsin.Though most of the substrates are suitable to chymotrypsin, Ala-Ala-Phe-p-NAis the best.

Key words: chromogenic substrate, Synthesis, Kinetics, Chymotrypsin

TrendMD: