高等学校化学学报

高等学校化学学报 ›› 2012, Vol. 33 ›› Issue (10): 2213.doi: 10.7503/cjcu20111157

• 分析化学 • 上一篇    下一篇

基于阳离子型共轭聚合物和核酸适体的腺苷检测新方法

黄艳琴, 秦伟胜, 任厚基, 曹国益, 刘兴奋, 黄维   

  1. 南京邮电大学信息材料与纳米技术研究院, 有机电子与信息显示国家重点实验室培育基地, 南京 210046
  • 收稿日期:2011-12-06 出版日期:2012-10-10 发布日期:2012-09-12
  • 通讯作者: 黄艳琴, 女, 博士, 副教授, 主要从事有机/高分子功能材料和化学生物传感研究. E-mail: iamyqhuang@njupt.edu.cn E-mail:iamyqhuang@njupt.edu.cn
  • 基金资助:

    国家"九七三"计划项目(批准号: 2009CB930601, 2012CB933301); 国家自然科学基金(批准号: 20804018, 51073078, 21005040); "有机与生物光电子学"教育部创新团队项目(批准号: IRT1148); 江苏高校优势学科建设工程项目和江苏省高校自然科学研究面上项目(批准号: 10KJB150014)资助.

Novel Adenosine Detection Method Based on Cationic Conjugated Polymer and Aptamer

HUANG Yan-Qin, QIN Wei-Sheng, REN Hou-Ji, CAO Guo-Yi, LIU Xing-Fen, HUANG Wei   

  1. Key Laboratory for Organic Electronics & Information Displays and Institute of Advanced Materials, Nanjing University of Posts and Telecommunications, Nanjing 210046, China
  • Received:2011-12-06 Online:2012-10-10 Published:2012-09-12

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被引次数

5

摘要：

建立了一种基于阳离子型共轭聚合物和核酸适体的腺苷检测新方法. 荧光素修饰的短链DNA与腺苷的核酸适体部分互补, 形成双链DNA; 阳离子型共轭聚合物通过静电作用与双链DNA结合, 发生高效率的荧光共振能量转移(FRET). 加入腺苷后, 腺苷与核酸适体发生特异性结合, 导致双链DNA分解成单链, 使静电吸引力下降, 能量转移效率降低. 通过阳离子型共轭聚合物对单双链DNA的高效识别, 可快速简易地检测出腺苷.

关键词: 水溶性共轭聚合物, 核酸适体, 腺苷, 荧光检测, 荧光共振能量转移

Abstract:

We designed a novel adenosine detection system based on cationic conjugated polymer (CCP) and aptamer. A fluorescein-modified short-chain DNA can partially hybridize with adenosine aptamer to form a double stand DNA(dsDNA). The CCP associated with the dsDNA by electrostatic attractions and an efficient fluorescence resonance energy transfer (FRET) from the CCP to the fluorescein occurred. When adenosine was added, it bonded specifically to the aptamer, making the dsDNA break into single stand DNA(ssDNA), then the FRET efficiency decreased. Thus, we could detect the adenosine quickly and easily through the high signal discrimination between dsDNA and ssDNA with the CCP. The results show that the degree of FRET efficiency decrease is related to the concentration of adenosine. This detection method has the advantages including easy operations, low costs, and relatively high sensitivity; moreover, it can be extended to detections of other targets owning aptamers.

Key words: Water-soluble conjugated polymer, Aptamer, Adenosine, Fluorescence detection, Fluorescence resonance energy transfer

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