高等学校化学学报 ›› 2012, Vol. 33 ›› Issue (10): 2205.doi: 10.7503/cjcu20111026

• 分析化学 • 上一篇    下一篇

维生素B12与12-钨磷酸相互作用的双波长共振瑞利散射光谱及其分析应用

崔志平, 刘绍璞, 刘忠芳, 胡小莉   

  1. 发光与实时分析教育部重点实验室, 西南大学化学化工学院, 重庆 400715
  • 收稿日期:2011-11-03 出版日期:2012-10-10 发布日期:2012-09-12
  • 通讯作者: 胡小莉, 女, 教授, 主要从事分子光谱研究. E-mail: xiaolihu@swu.edu.cn E-mail:xiaolihu@swu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号: 20875078)和重庆市科委重点实验室专项经费资助.

Dual-wavelength Overlapping Resonance Rayleigh Scattering Spectra of the Interactions of Vitamin B12 with 12-Tungstophosphoric Acid and Their Analytical Applications

CUI Zhi-Ping, LIU Shao-Pu, LIU Zhong-Fang, HU Xiao-Li   

  1. Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China
  • Received:2011-11-03 Online:2012-10-10 Published:2012-09-12

摘要:

建立了一种双波长共振瑞利散射光谱测定维生素B12的新方法. 在pH=1.0的HCl介质中, 维生素B12(VB12)与12-钨磷酸(TP)形成摩尔比为3:1的离子缔合物, 导致双波长共振瑞利散射(DWO-RRS)、 二级散射(SOS)和倍频散射(FDS)光谱显著增强, 其最大散射波长分别位于330和370 nm(RRS), 608 nm(SOS)和386 nm(FDS). 在一定范围内, 3种散射增强(ΔIRRS, ΔISOS和ΔIFDS)均与VB12的浓度成线性关系. 该方法具有较高的灵敏度, RRS, SOS和FDS法对VB12的检出限(3σ)在2.0~7.6 ng/mL之间. 研究了反应条件和共存物质的影响, 结果表明, 该方法具有良好的选择性. 据此, 提出了简便、 快速、 准确且高灵敏测定痕量VB12的光散射新方法, 适用于片剂和尿样中VB12的测定. 还对反应机理和散射光谱增强的原因进行了讨论.

关键词: 维生素B12, 12-钨磷酸, 双波长共振瑞利散射, 二级散射, 倍频散射

Abstract:

A new dual-wavelength overlapping resonance Rayleigh scattering (DWO-RRS) method was developed to detect vitamin B12(VB12) with 12-tungstophosphoric acid(TP). In a HCl medium(pH=1.0), VB12 could react with TP to form a 3: 1 ion-association complex. As a result, the new spectra of DWO-RRS, second order scattering(SOS) and frequence doubling scattering(FDS) appeared and their intensities were enhanced greatly. Their maximum wavelengths were located at 330 nm(RRS), 370 nm(RRS), 608 nm(SOS) and 386 nm(FDS), respectively. The three kinds of scattering intensities(ΔIRRS, ΔISOS and ΔIFDS) were proportional to the concentration of VB12 in certain ranges. The methods had high sensitivity with the detection limit of 2.0-7.6 ng/mL. The optimal conditions and the effects of coexistent substances of RRS method were investigated. So, a simple, rapid and new method had been proposed to determine trace VB12. It had been applied to the determination of VB12 in urine samples and vitamin B12 tablets with satisfactory results. In addition, the reasons for RRS enhancement and composition of ion-association complex were discussed.

Key words: Vitamin B12, 12-Tungstophosphoric acid, Dual-wavelength overlapping resonance Rayleigh scattering, Second order scattering, Frequence doubling scattering

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