Preparation of Cibacron Blue F3GA Attached Chitosan Microspheres and Their Adsorption Properties for Bovine Serum Albumin(BSA)

Chem. J. Chinese Universities ›› 2005, Vol. 26 ›› Issue (12): 2363.

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Preparation of Cibacron Blue F3GA Attached Chitosan Microspheres and Their Adsorption Properties for Bovine Serum Albumin(BSA)

ZHANG Jing¹, ZHANG Zheng-Pu^1*, SONG Yu¹, HE Bing-Kun²

1. Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, Nankai University, Tianjin 300071, China;
2. Research Center for New Drugs, North China Pharmaceutical Corporation, Shijiazhuang 050015, China

Received:2004-12-07 Online:1905-03-14 Published:1905-03-14
Contact: ZHANG Zheng-Pu;E-mail:zhangzp@nankai.edu.cn

Abstract

Abstract:

A series of novel aminated chitosan microbeads were prepared from chitosan through inverse suspension crosslinking, hydroxylpropyl-chlorination and amination. Affinity dye-ligand, Cibacron Blue F3GA, was covalently coupled with the aminated chitosan microspheres via space arm between the triazine ring of Cibacron Blue F3GA and the amino groups of the microspheres. The maximum attachment of Cibacron Blue F3GA was 494 μmol/g by changing the crosslinking degree of the microspheres. Albumin adsorption onto Cibacron Blue F3GA-attached chitosan microspheres was investigated. The BSA equilibrium adsorption capacity was 95.2 mg/g dry beads. The adsorption phenomena appeared to follow a typical Langmuir isotherm. The highest desorption ratio(over 99%) was achieved by using 1.0 mol/L NaSCN(pH=8.0). The Cibacron Blue F3GA-attached chitosan microspheres could be reused without significant decreases in the adsorption capacities.

Key words: Chitosan; Cibacron Blue F3GA; Dye-affinity sorbent; BSA, Chitosan, Cibacron Blue F3GA, Dye-affinity sorbent, BSA

CLC Number:

O631
O647.33

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ZHANG Jing, ZHANG Zheng-Pu, SONG Yu, HE Bing-Kun. Preparation of Cibacron Blue F3GA Attached Chitosan Microspheres and Their Adsorption Properties for Bovine Serum Albumin(BSA)[J]. Chem. J. Chinese Universities, 2005, 26(12): 2363.

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Preparation of Cibacron Blue F3GA Attached Chitosan Microspheres and Their Adsorption Properties for Bovine Serum Albumin(BSA)

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