Preparation and Characterization of Graphene Oxide-sodium Alginate-chitosan Composite Scaffold

doi:10.7503/cjcu20200217

Abstract

Abstract:

The composite scaffold material was constructed by freeze-drying technology from graphene oxide, sodium alginate and chitosan. The effects of graphene oxide content on the microstructure, porosity, swelling ratio, in vitro degradation performance, mechanical properties and biocompatibility of the scaffold materials were studied to determine the optimal graphene oxide content in the composite scaffold. The results show that the composite material has a solid sponge-like structure with a certain morphological plasticity. Scanning electron microscopy(SEM) observations show that each group of supports has a three-dimensional network structure. As the content of graphene oxide increased, the pore size gradually decreased and the pore wall thickness increased, the swelling ratio and degradation rate of the composite scaffold gradually decreased while the mechanical strength was significantly enhanced. Results of in vitro cytotoxicity showed that when the graphene oxide content was 0.3%(mass fraction), the cell survival rate was the highest, and when the graphene oxide content increased, the cell activity was significantly inhibited, causing cell death. Finally, the optimal content of graphene oxide in the composite scaffold is 0.3%. Our research lays a good foundation for the application of graphene oxide in bone tissue engineering materials.

Key words: Scaffold, Graphene oxide, Sodium alginate, Chitosan

CLC Number:

O636

TrendMD:

WANG Bowei, MA Rui, WU Fan, LIU Zhihui, LI Lingfeng, ZHANG Xiao, LIU Dingkun, YANG Nan, LI Meihui, YANG Defeng, SUN Qi. Preparation and Characterization of Graphene Oxide-sodium Alginate-chitosan Composite Scaffold[J]. Chem. J. Chinese Universities, 2020, 41(9): 2099.

Figures/Tables 10

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Cytotoxicity level	Relative growth rate(RGR, %)	Evaluation
0	≥100	Qualified
1	75—99	Qualified
2	50—74	Valuation combined with the cell shape
3	25—49	Disqualified
4	1—24	Disqualified
5	0	Disqualified

Cytotoxicity level	Relative growth rate(RGR, %)	Evaluation
0	≥100	Qualified
1	75—99	Qualified
2	50—74	Valuation combined with the cell shape
3	25—49	Disqualified
4	1—24	Disqualified
5	0	Disqualified

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