Preparation of Modified Chitosan Antibacterial Nanospheres†

doi:10.7503/cjcu20180351

Abstract

Abstract:

A novel antibacterial nanospheres was prepared by modification of chitosan with N-halamine, and characterized by nuclear magnetic resonance(NMR) and ultraviolet spectrum(UV). The effect of concentration and volume ratio of CS-HDH, polysodium tripolyphosphate(TPP) and the volume ratio of the two polymers on the formation and particle size of nanoparticles were discussed. The antibacterial properties were also measured. The results show that the nanospheres has a stable shape and uniform distribution of particle size. Excellent antibacterial properties can be obtained, and 10⁷ cfu(colong-forming units) of S. aureus and E. coli can be killed within 30 min.

Key words: Chitosan, N-Halamine, Nanospheres, Antibacterial

CLC Number:

O636.9

TrendMD:

YIN Maoli,WANG Yingfeng,REN Xuehong. Preparation of Modified Chitosan Antibacterial Nanospheres^†[J]. Chem. J. Chinese Universities, 2019, 40(1): 173.

Figures/Tables 8

Scheme 1 Synthesis of CS-HDH

Scheme 2 Schematic diagram of ionic cross-linking between CS-HDH and TPP

Fig.1 UV spectra of CS(a), HDH(b), CS-HDH(c)(A) and standard curve of HDH(B)

Table 1 Different concentrations of CS-HDH and TPP on effect of liquid properties*

c(CS-HDH)/(mg·mL^-1)	c(TPP)/(mg·mL^-1)
c(CS-HDH)/(mg·mL^-1)	0.8	1.0	1.2	1.5	2.0
1.0	O	P	P	P	P
1.5	O	O	P	P	P
2.0	O	O	O	O	P
2.5	O	O	O	O	O
3.0	C	O	O	O	O
3.5	C	O	O	O	O
4.0	C	O	O	O	O

Fig.2 Influence of CS-HDH(A), TPP(B) concentration and TPP/CS-HDH volume ratio on the nanospheres size(C) and the lognormal distribution of nanospheres size(D)

Fig.3 SEM images of CS-HDH nanospheres in 10000 magnification(A) and 50000 magnification(B)

Table 2 Antibacterial property against S. aureus and E. coli O157:H7

Sample	Contact time/min	S. aureus^a		E. coli O157:H7^b
Sample	Contact time/min	Mortality	lg(Mortality)	Mortality	lg(Mortality)
CS	60	20.59	0.10	28.89	0.15
CS-HDH	60	16.18	0.08	23.58	0.13
CS-HDH	5	26.48	0.14	10.72	0.06
Nanospheres	10	44.12	0.26	42.86	0.25
	30	57.36	0.37	74.29	0.60
	60	87.06	0.87	85.72	0.85
Chlorinated CS-HDH	5	86.77	0.88	95.78	1.38
Nanospheres	10	93.98	1.22	97.11	1.54
	30	100.00	7.83	100.00	7.65
	60	100.00	7.83	100.00	7.65

Fig.4 Photographs of the antibacterial efficacy against bacteria culture plates of S. aureus upon 60 min contact with CS(A), CS-HDH(B), CS-HDH nanospheres(C), chlorinated CS-HDH nanospheres(D) and upon 5 min(E), 10 min(F), 30 min(G) contact with chlorinated CS-HDH nanospheres

References 15

[1]	Wang A.Q., Chitin Chemistry, Science Press, Beijing, 2007
	(王爱勤. 甲壳素化学. 北京: 科学出版社, 2007)
[2]	Guo M.M., Study on Applications of Chitosan in Antimicrobial Food Packaging, Jiangnan University, Wuxi, 2015
	(郭鸣鸣. 壳聚糖在几种食品抗菌包装中的应用研究. 无锡: 江南大学, 2015)
[3]	Cruz-Romero M. C., Food Control, 2013, 34, 393—397
[4]	Sui S.Y., Study on Whey Protein Concentrate-chitosan Antibacterial Composite Edible Films, Jilin University, Changchun, 2015
	(隋思瑶. 浓缩乳清蛋白-壳聚糖抗菌复合膜的研究. 长春: 吉林大学, 2015)
[5]	Williams D. E., Elder E. D., Worley S. D., Applied and Environmental Microbiology,1988, 54(10), 2583—2585
[6]	Liu Y., Liu Y., Ren X. H., Huang T. S., Applied Surface Science,2014, 296, 231—236
[7]	Jiang Z. M., Ma K. K., Du J. M., Li R., Ren X. H., Huang T. S., Applied Surface Science,2014, 288, 518—523
[8]	Li R., Hu P., Ren X. H., Worley S. D., Huang T. S., Carbohydrate Polymers,2013, 92, 534—539
[9]	Cheng X. L., Ma K. K., Li R., Ren X. H., Huang T. S., Applied Surface Science,2014, 309, 138—143
[10]	Yin M. L., Chen X. L., Li R., Huang D., Fan X. Y., Ren X. H., Huang T. S., Journal of Applied Polymer Science,2016, 133(46), .44204
[11]	Wang Y. F., Li L., Liu Y., Ren X. H., Liang J., Materials Science and Engineering C,2016, 69, 1075—1080
[12]	Wu Z. Y., Chen Z., Huang H., Tian P. P., Journal of Nantong University(Natural Science Edition), 2005, 25, 20—22
	(吴振宇, 陈钟, 黄华, 田鹏鹏. 南通大学学报(自然科学版), 2005, 25, 20—22)
[13]	Gierszewska-Dru<inline-formula><mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="Mml4-0251-0790-40-1-173"><mml:mover accent="true"><mml:mi>z</mml:mi><mml:mtext fontstyle="italic">˙</mml:mtext></mml:mover></mml:math></inline-formula>y<inline-formula><mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="Mml5-0251-0790-40-1-173"><mml:mover><mml:mrow><mml:mi mathvariant="normal">n</mml:mi></mml:mrow><mml:mrow><mml:mi>'</mml:mi></mml:mrow></mml:mover></mml:math></inline-formula>ska M., Ostrowska-Czubenko J., Polish Chitin Society,2011, 16, 15—22
[14]	Qi L. F., Xu Z. R., Jiang X., Hu C. H., Zou X. F., Carbohydrate Research,2004, 339, 2693—2700
[15]	Wang Y. F., Liu Y., Tian H. R., Zhai Y. S., Pan N. Y., Yin M. L., Ren X. H., Liang J., Colloid and Polymer Science,2017, 295(910), 1897—1900