Preparation and Properties of CS/nHA Porous Composite Scaffold Based on In-situ Precipitation Method†

doi:10.7503/cjcu20180724

Abstract

Abstract:

The initial porous scaffold containing calcium phosphate precursors(CaP) was obtained by in situ precipitation and freezing phase separation techniques. The chitosan(CS) membranes generated in situ on the outer layer of porous scaffolds were used to slow down the penetration rate of OH^- ions in NaOH solution, so as to achieve the purpose of slow formation of nano-hydroxyapatite(nHA), and thus to prepare CS/nHA porous composite scaffolds with uniform distribution of nHA. The scanning electron microscopy(SEM) and universal testing machine were used to study the structure and properties of the composite scaffold. It was found that the nHA was acicular structure, with a length of 80―200 nm and a width of 20―50 nm. With the increase of nHA content, the porosity of the composite scaffold decreased from (93.8±3.3)% to (87.7±3.8)%, and the compressive strength gradually increased from (0.5±0.09) MPa to (1.5±0.06) MPa. No obvious agglomeration of nHA was found, and nHA was evenly distributed in the CS matrix when the content of nHA in the composite scaffold was 25%. It is concluded by infrared spectrometer(FTIR), X-ray diffractometer(XRD) and X-ray photoelectron spectrometer(XPS) analysis that there may be coordination and hydrogen bonding between nHA and CS. The results of cell experiments showed that the nHA/CS porous composite scaffold has good biocompatibility, and the cells adhered to the scaffold. It is expected that the CS/nHA porous composite scaffold has a good application prospect in the field of bone tissue engineering.

Key words: In situ precipitation, Nano-hydroxyapatite, Chitosan, Bone tissue engineering, Porous composite scaffold

CLC Number:

O636

TrendMD:

WANG Ruanfeng,YAN Shifeng,HU Zhen,YIN Jingbo. Preparation and Properties of CS/nHA Porous Composite Scaffold Based on In-situ Precipitation Method^†[J]. Chem. J. Chinese Universities, 2019, 40(5): 1080.

Figures/Tables 13

Scheme 1 Preparation of CS/nHA composite scaffold

Fig.1 Pictures of scaffolds(A) Initial scaffold; (B) CS membrane-covered scaffold; (C) crosssection of CS membrane-covered scaffold;(D) CS/nHA composite scaffold.

Fig.2 FTIR spectra of composite scaffold

Fig.3 XRD patterns of composite scaffolds

Scheme 2 Schematic illustration of bonding between CS and nHA

Fig.4 XPS of CS scaffold(A, C) and CS/nHA25 scaffold(B, D) (A, B) N1s; (C, D) O1s.

Fig.5 TGA of composite scaffolds(A) and theoretical and measured values of nHA content in composite scaffolds(B)

Fig.6 SEM images of composite scaffolds(A) CS scaffold; (B) CS/nHA5 scaffold; (C) CS/nHA15 scaffold; (D) CS/nHA25 scaffold.

Fig.7 SEM image(A), EDS mappings(B, C) and high-magnification SEM image(D) and EDS analysis(inset) of CS/nHA25 scaffold

Fig.8 Porosity(A) and water uptake and retention levels(B) of composite scaffolds

Fig.9 Compression strength of composite scaffolds(A) and shape retention of composite scaffolds as a function of scaffold immersion time in SBF(B)

Fig.10 Live/dead staining images of rASCs cells cultured on CS/nHA25 scaffold for 1 d(A), 4 d(B), 7 d(C) and MTT assay for proliferation(D)

Fig.11 LSCM images of rASCs cells adhered to CS/nHA25 scaffold after 7 d of seeding Scaffold and cell nuclei stained blue(A) while the Actin filament stained red(B); (C) represented merged images of the two fluorochromes for the samples.

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