Preparation and Characterization of Chitosan Alginate Plastic Scaffolds†

doi:10.7503/cjcu20170565

Abstract

Abstract:

Based on anion and cation electrostatic load principle, using sodium alginate and chitosan as raw materials, sustained-release microspheres were obtained by the drip method and layer-by-layer self-assembly. The microspheres were then put into a certain percentage of sodium alginate-chitosan solution, and a new kind of scaffold with microspheres embedded in the spongy structure called sustained-release microsphere-type scaffolds was obtained. The influences of the component ratio of the sustained-release microspheres on the porosity, contraction, hydrophilicity and degradation properties of the sustained-release microsphere-type scaffolds were investigated. The pore size was between 140 and 200 μm analyzed by scanning electron microscopy(SEM). There was no difference in the cytotoxicity text of the scaffold’s leachate between the experimental group and the control group. When the component ratio of microspheres was 10%, the scaffolds had the highest porosity ratio(68.2%—70.8%), the lowest contraction percentage(4.4%—5.2%) and the best hydrophilicity. The less microspheres the scaffolds had, the faster the degradation rate was. For preventing the degradation rate too fast and carrying more medicines, the scaffolds, whose component ratio of microspheres was 20%, are much better. The microsphere-type scaffold material has good plasticity before freeze-drying. The microsphere-type scaffolds provided an example of the combination with sustained-release systems and existing porous scaffold materials.

Key words: Tissue engineering scaffold, Sodium alginate, Chitosan, Sustained-release microsphere

CLC Number:

O636

TrendMD:

LIU Zhihui,QIU Tianyuan,DU Liuyi,YANG Junxing,LIU Kang,WANG Bowei. Preparation and Characterization of Chitosan Alginate Plastic Scaffolds^†[J]. Chem. J. Chinese Universities, 2018, 39(5): 1105.

Figures/Tables 10

Fig.1 FTIR spectra of scaffolds Note:a. SA; b. CS; c. CA-CS-SA-CS microspheres; d. scaffolds without microspheres; e. scaffolds with microspheres.

Fig.2 Optical photos of frontal view(A) and side view(B) of the scaffolds

Fig.3 SEM images of the scaffolds Note:(A) Microsphere; (B) edge of left microsphere and right spongy organization; (C) surface of spongy organization; (D) internal structure of spongy organization.

Fig.4 Porosity ratio of scaffolds with different component ratio of microspleres

Fig.5 Contraction percentage of scaffolds with different component ratio of microspleres

Fig.6 Swelling ratio of scaffolds with different component ratio of microspheres

Fig.7 Degradation of scaffolds with different component ratio of microspheres

Fig.8 Degradation of scaffolds with 30% microspheres in different time Note:Time/d:(A) 1; (B) 5; (C) 10; (D) 15; (E) 20.

Fig.9 Modulus of scaffolds with different component ratio of microspheres

Fig.10 Cytotoxicity test by compare growth of the cells under the medium with(a) and without(b) leachate of scaffolds

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