Preparation of Cu2+-Chitosan Quaternary Ammonium Salt-g-Poly(acrylicacid) with Efficient Antibacterial Activity

doi:10.7503/cjcu20160626

Abstract

Abstract:

Acrylic acid(AAc) was grafted to chitosan quaternary ammonium salt(HACC) by ammonium persulfate as an initiator. HACC-g-PAAc-Cu²⁺ with efficient antibacterial activity was further prepared through complexation of Cu²⁺ with HACC-g-PAAc. The chemical structure of the grafted HACC was characterized by Fourier transform infrared spectroscopy and proton nuclear magnetic resonance(¹H NMR). The capacity of grafted HACC loading Cu²⁺ was determined by ultraviolet-visible spectroscopy(UV-Vis) and thermagravimetric analysis(TGA). Antimicrobial property of HACC-Cu²⁺ and HACC-g-PAAc-Cu²⁺ against staphylococcus aurous(S. Aurous) and E. coli bacillus were tested. Toxicity for oral intake of mice and coating irritation on rabbit skin of HACC-Cu²⁺ and HACC-g-PAAc-Cu²⁺ were also evaluated. The property of antibacterial and capacity of complexing Cu²⁺ can be improved by grafting of AAc. The molar ratio of Cu²⁺/HACC increased from 3:7 before grafting to 1:1 after grafting. The minimum inhibitory concentration(MIC) of S. Aurous and E. coli bacillus decreased from 60 mg/L to 9.2 mg/L and 37 mg/L to 6.3 mg/L. Moreover, HACC-Cu²⁺ and HACC-g-PAAc-Cu²⁺ have no toxic ingestion and skin irritation.

Key words: Chitosan quaternary ammonium salt, Cupric ion, Antibacterial property, Acrylic acid

CLC Number:

O636.9

TrendMD:

LI Hui, XING Zhe, LI Rong, ZHANG Mingxing, WANG Minglei, TAN Yizhe, WU Guozhong. Preparation of Cu²⁺-Chitosan Quaternary Ammonium Salt-g-Poly(acrylicacid) with Efficient Antibacterial Activity[J]. Chem. J. Chinese Universities, 2017, 38(5): 902.

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Preparation of Cu²⁺-Chitosan Quaternary Ammonium Salt-g-Poly(acrylicacid) with Efficient Antibacterial Activity

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