Development and Performance of Laser-induced Fluorescence Microfluidic Chip Analyzer

Abstract

Abstract: A laser induced fluorescence microfluidic chip system was developed. The detection system was designed according to the confocal principle. Main features of the system include the alternated emission filter in order to select many dyes, and computer controlled focusing Via 3 D micro manipulator and charge coupled device(CCD), and two curves of the current vs. time and two curves of the voltage vs. time for injection channel and separation channel displayed on the computer′s screen. The detection limit reached 6.67×10^-13 mol/L for rhodamine 6G. The linear range of detection was 4.02×10^-6 mol/L～4.02×10^-9mol/L. The high performance of the system was demonstrated by separation and detection of DNA fragments and PCR products on a home made cast molded PMMA microchip.

Key words: Laser induced fluorescence spectrometer, Microfluidic chip, Microfluidic chip analyzer

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ZHOU Xiao-Mian, DAI Zhong-Peng, LIN Bing-Cheng . Development and Performance of Laser-induced Fluorescence Microfluidic Chip Analyzer[J]. Chem. J. Chinese Universities, 2004, 25(3): 436.

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Development and Performance of Laser-induced Fluorescence Microfluidic Chip Analyzer

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