Fabrication of Bionic Leaf Model and Its Application in Agarose Microfluidic Chip

doi:10.7503/cjcu20220445

Abstract

Abstract:

The vein sequence of leaf is similar with human vascular network in terms of structure and function， highlighting a manner to create human vessel-like structure in vitro. However， several technical（irreproducible vein pattern of different leaves） and logistic（limited availability across seasons） issues prevent the use of fresh leaves as template. Herein， a practical and effective method for biomimetically replicating the leaf structure was developed and this bionic leaf model was applied to constructing a low-cost and simple-to-operate agarose microfluidic chip. Moreover， the number and size of the pulse sequences（channels） of the primary veins， secondary veins and tertiary veins in the agarose chip were measured， where maximum and minimum widths of pulse sequence are 1038.02 μm and 36.32 μm， respectively. Lastly， the fabricated channels of different widths provided stable and reliable gradients for studying bacterial chemotaxis. Taken together， our methodology is of great significance for drug screening and microbial research.

Key words: Bionics, Microfluidic chip, Chemotaxis

CLC Number:

O657

TrendMD:

WANG Fangyuan, ZHANG Fenxian, LI Yi, GAO Jianhua, NIU Yanbing, SHEN Shaofei. Fabrication of Bionic Leaf Model and Its Application in Agarose Microfluidic Chip[J]. Chem. J. Chinese Universities, 2022, 43(11): 20220445.

Figures/Tables 7

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