Chem. J. Chinese Universities ›› 2014, Vol. 35 ›› Issue (1): 30.doi: 10.7503/cjcu20130854

• Analytical Chemistry • Previous Articles     Next Articles

Detection of Inhibitory Effects of Suramin and Cisplatin on Hepatocellular Carcinoma Cells Using Surface Plasmon Resonance Imaging

XIE Fei, ZHANG Ying, JIN Wei, PAN Tingting, ZHOU Chao, REN Hao, JIN Qinhan, MU Ying*()   

  1. Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310058
  • Received:2013-09-02 Online:2014-01-10 Published:2013-12-05
  • Contact: MU Ying E-mail:muying@zju.edu.cn
  • Supported by:
    † Supported by the National Natural Science Foundation of China(Nos.31070772, 31270907) and the Science and Technology Programs of Zhejiang Province(No.2011C37029)

Abstract:

A rapid, label-free and novel evaluation of anti-cancer drug efficacy was proposed via surface plasmon resonance imaging(SPRi). A microfluidic chip with micro-posts was designed and fabricated in polydimethylsiloxane(PDMS) to work as a continuous flow cell. Specific entrapment and detection of tumor cells were accomplished by a homemade SPRi system with the microfluidic chip. On this basis, the inhibitory effects of suramin and cisplatin on the proliferation of HepG2 cells was investigated. Meanwhile, we used auxiliary cell counting kit-8(CCK-8) assay to examine cell viability after drug incubation. The results of the SPRi method showed that both suramin and cisplatin could inhibit the proliferation of HepG2 cells in a dose-dependent and time-dependent manner.

Key words: Surface plasmon resonance imaging, Microfluidic chip, CCK-8 assay, Suramin, Cisplatin

CLC Number: 

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