Microfluidic Cell Chip Analytical System with LED Induced Fluorescence Detector

doi:10.3969/j.issn.0251-0790.2012.01.008

Abstract

Abstract: Based on micro-electro-mechanical systems technique and microfluidic cell chip analytical metho-dology, a microfluidic cell chip analytical system with light emitting diode(LED) induced fluorescence detector was constructed for monitoring cells in the suspensions with a low concentration below 40 Cell/mL. Cells passed through the micro-channels on the designed microchip, and the target cells labeled by 5- or 6-(N-succinimidyloxycarbonyl)-3',6'-O,O'-diacetylfluorescein(CFDA SE) were stimulated by the LED to produce fluorescence signals which were collected by a photomultiplier tube from the roof window and changed into electrical signals finally. The HepG2 hepatoma cells labeled by CFDA SE in samples could be detected and counted by this analytical microsystem. The designed microsystem ran effectively with the coexistence of blood cells. The whole microsystem is simple, convenient and high sensitivity.

Key words: Microfluidic chip, HepG2 hepatoma cell, Cell detection, Microsystem with light emitting diode(LED) induced fluorescence detector

CLC Number:

O657

TrendMD:

LI Dong-Shun, XU Yi, PENG Jin-Lan, ZHOU Zhen, GAN Jun, WANG Yao. Microfluidic Cell Chip Analytical System with LED Induced Fluorescence Detector[J]. Chem. J. Chinese Universities, 2012, 33(01): 49.

References

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