Fabrication of Nylon Membrane-based Microfluidic Chips and Its Application in Color Sensing of Glucose

doi:10.7503/cjcu20120349

Chem. J. Chinese Universities ›› 2012, Vol. 33 ›› Issue (11): 2405.doi: 10.7503/cjcu20120349

• Analytical Chemistry • Previous Articles Next Articles

Fabrication of Nylon Membrane-based Microfluidic Chips and Its Application in Color Sensing of Glucose

YANG Jun^1,2, QI Li¹, MA Hui-Min¹, CHEN Yi¹

1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China

Received:2012-04-13 Online:2012-11-10 Published:2012-10-15

Abstract

Abstract:

A new type of nylon membrane-based microfluidic chips was fabricated by transferring the positive photoresist onto the thin membrane material via photolithography. It was found that the fabrication period can be controlled in 1 h with substantive production, and the whole fabrication processes was simplified and improved. Meanwhile, by fixing horseradish peroxidase and glucose oxidase on the membrane chips, glucose samples were assayed at different concentrations based on the two-steps enzymatic reactions. Additionally, the obviously changed color corresponding to the gradient concentration of glucose shows that the new type membrane chips could be used extensively in practice in the future.

Key words: Nylon membrane, Photolithography, Microfluidic chip, Glucose

CLC Number:

O657

TrendMD:

YANG Jun, QI Li, MA Hui-Min, CHEN Yi. Fabrication of Nylon Membrane-based Microfluidic Chips and Its Application in Color Sensing of Glucose[J]. Chem. J. Chinese Universities, 2012, 33(11): 2405.

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Fabrication of Nylon Membrane-based Microfluidic Chips and Its Application in Color Sensing of Glucose

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