Chem. J. Chinese Universities ›› 2020, Vol. 41 ›› Issue (6): 1243.doi: 10.7503/cjcu20200080

• Analytical Chemistry • Previous Articles     Next Articles

On-chip Sorting of Beads with Different Magnetic Responsiveness by Lateral Magnetophoresis

ZHANG Zuoran,ZHANG Li,ZHANG Zhiling*()   

  1. College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China
  • Received:2020-02-17 Online:2020-06-10 Published:2020-03-17
  • Contact: Zhiling ZHANG E-mail:zlzhang@whu.edu.cn
  • Supported by:
    † National Natural Science Foundation of China(21775111)

Abstract:

Magnetic separation is widely used in biochemical analysis. The separations of multiple small targets by magnetic force still remain a challenge. To solve the problem, different types of magnetic probes coupled with small targets have to be sorted. Here, we propose a strategy to separate magnetic nano-beads(MNs) with either a strong or weak magnetic responsiveness on a chip system. The sorting principle is utilizing the lateral magnetophoresis of MNs. A theoretical model is proposed to analyze the lateral displacement of MNs including aggregation and deflection. On this basis, chips were designed to separate MNs. By adjusting the flow rates, MNs with different magnetic responsiveness were sequentially separated. The sorting efficiency is acceptable and the procedure is easy to perform. MNs with strong magnetic responsiveness can be completely separated, which is valuable for rare targets sorting. The system was applied to sort DNA from hepatitis B virus(HBV) and complementary DNA from hepatitis C virus(HCV) simultaneously. This system has the potential for wide application in biochemical analysis.

Key words: Microfluidic chip, Magnetic nanospheres, Magnetic separation

CLC Number: 

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