Abstract:

Taking advantage of the ultraviolet polymerization characteristic of poly(ethylene glycol) diacrylate(PEGDA) hydrogel material, a double-layer hydrogel microfluidic chip was fabricated rapidly by photopolymerization method. Moreover, feasibility of the chip culture for tumor cell metabolism detection was validated. The hydrogel material with better biocompatibility and handleability compared to traditional microfluidic chip material could be directly processed to obtain desirable micro-structure. The experimental results showed that the proposed method could achieve the fabrication of double-layer cell culture and detection chip with short time treatment. Cells could be well captured and cultured in the hydrogel microfluidic chip with excellent activity. Chip integrated with porphyrin colorimetric sensor array system could effectively distinguish different types of tumor cell by metabolism characteristics detection. The hydrogel microfluidic chip has the potential of practicable application once large-scale preparation is accomplished.

Key words: Hydrogel microfluidic chip, Poly(ethylene glycol) diacrylate(PEGDA), UV photopolymerization, Tumor cell metabolites detection