Spectroscopic and Electrochemical Studies on the Enzyme-catalyzed Reaction of PAP-H2O2-HRP

Abstract

Abstract: The pure product of p-aminophenol(PAP)-H₂O₂-horse peroxidase(HRP) of H₂O₂ oxidizing PAPcatalyzed by HRPwas prepared with chemical method. The enzyme-catalyzed reaction was investigated by electroanalytical chemistry, UV-Vis, IR,¹³C NMR, ¹H NMR, mass spectrum and elemental analysis etc.. Under the selected enzyme-catalyzed reaction conditions, the oxidation product of PAPwith H₂O₂ catalyzed by HRPis 3,4-di-[(4-hydroxyphenyl)amino]-6-[(4-hydroxyphenyl)imino]-2,4-cyclo-hexadiene-1-one.

Key words: p-Aminophenol, Horseradish peroxidase(HRP), Voltammetry, Enzyme-catalysis

CLC Number:

O657.14

TrendMD:

ZHANG Shu-Sheng, JIAO Kui, LI Hui-Jing, CHEN Hong-Yuan . Spectroscopic and Electrochemical Studies on the Enzyme-catalyzed Reaction of PAP-H₂O₂-HRP[J]. Chem. J. Chinese Universities, 2000, 21(5): 710.

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Spectroscopic and Electrochemical Studies on the Enzyme-catalyzed Reaction of PAP-H₂O₂-HRP

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