Research on the Electrochemical Activity of Magnetospirillum Magneticum AMB-1†

doi:10.7503/cjcu20150240

Abstract

Abstract:

The electrochemical activity of Magnetospirillum magneticum AMB-1 was investigated using cyclic voltammetry and chronoamperometry. Compared with the cyclic voltammograms in different culture conditions, AMB-1 exhibited a good extracellular electron transfer capability after being cultured for 3 d. The oxidation peak was observed at 0.1 V and reduction peak at -0.2 V. The increase of dissolved oxygen would cause a negative shift in oxidation peak(0 V) and a new reduction peak(-0.3 V) appeared. In addition, the formation of magnetosomes deadly inhibited the extracellular electron transfer of AMB-1. The results indicate that the formation of magnetosomes is concerned with extracellular electron transfer. The study is helpful to understand the mechanisms of biomineralization.

Key words: Magnetospirillum magneticum AMB-1, Magnetosome, Biomineralization, Electrochemical activity, Cyclic voltammetry

CLC Number:

O646

TrendMD:

WU Ranran, TIAN Xiaochun, WU Shenjian, LIU Yuangang, JIANG Yanxia, ZHAO Feng. Research on the Electrochemical Activity of Magnetospirillum Magneticum AMB-1^†[J]. Chem. J. Chinese Universities, 2015, 36(9): 1730.

Figures/Tables 8

Fig.1 TEM images of M. magneticum AMB-1 cells(A) Without Fe resource; (B) with only Fe3+; (C) with only Fe2+; (D) with both Fe3+ and Fe2+.

Fig.2 OD600(A) and Cmag(B) of AMB-1 in different culturing conditions

Fig.3 Cyclic voltammograms of M. magneticum AMB-1 and the ATCC medium(A) and i-t curve of AMB-1 with acetate addition(B)a. PBS+medium; b. AMB-1+PBS+medium.

Fig.4 Cyclic voltammograms of M. magneticum AMB-1 after being cultured for 2 d(a) and 3 d(b)

Fig.5 Cyclic voltammograms of AMB-1 under microaerobic(a) and aerobiotic conditions(b)

Fig.6 Current-time plots of AMB-1 on the general(a) and magnetic(b) GC electrodes(A) and photo of AMB-1 attached to the magnetic GC electrode marked in red cirde(B)

Fig.7 TEM images of M. magneticum AMB-1 cells attached on the magnetic GC(A), outside the magnetic GC(B) and in the solution with the general GC(C)

Fig.8 Cyclic voltammograms of M. magneticum AMB-1 attached on the magnetic GC(a), outside the magnetic GC(b) and in the solution with the general GC(c)

References 25

[1]	Blakemore R., Science, 1975, 190(4212), 377—379
[2]	Guerin W. F., Blakemore R. P., Appl. Environmen. Microb., 1992, 58(4), 1102—1109
[3]	Bazylinski D. A., International Microbiology, 2010, 2(2), 71—80
[4]	Frankel R.B., Dunin-Borkowski R. E., Posfai M., Bazylinski D. A., Handbook of Biomineralization: Biological Aspects and Structure Formation, Wiley-VCH Verlag GmbH, Weinheim, 2007, 126—144
[5]	Frankel R. B., Blakemore R. P., Wolfe R. S., Science, 1979, 203(4387), 1355—1356
[6]	Lower S. K., Hochella M. F., Beveridge T. J., Science, 2001, 292(5520), 1360—1363
[7]	Shi L., Rosso K. M., Zachara J. M., Fredrickson J. K., Biochem. Soc. Trans., 2012, 40, 1261—1267
[8]	Nakamura R., Kai F., Okamoto A., Newton G. J., Hashimoto K., Angew. Chem. Int. Ed., 2009, 48(3), 508—511
[9]	Cutting R. S., Coker V. S., Fellowes J. W., Lloyd J. R., Vaughan D. J., Geochim. Cosmochim. Ac., 2009, 73(14), 4004—4022
[10]	Carmona-Martinez A. A., Harnisch F., Fitzgerald L. A., Biffinger J. C., Ringeisen B. R., Schröder U., Bioelectrochemistry, 2011, 81(2), 74—80
[11]	He H., Yuan S. J., Tong Z. H., Huang Y. X., Lin Z. Q., Yu H. Q., Process Biochem., 2014, 49(2), 290—294
[12]	Heyen U., Schüler D., Appl. Microbiol. Biotechnol., 2003, 61(5/6), 536—544
[13]	Li K., Chen C., Chen C., Wang Y., Wei Z., Pan W., Song T., Enzyme Microb. Tech., 2015, 72, 72—78
[14]	Yang C. D., Takeyama H., Tanaka T., Matsunaga T., Enzyme Microb. Tech., 2001, 29(1), 13—19
[15]	Schüler D., Uhl R., Bäuerlein E., FEMS Microbiol. Lett., 1995, 132(1/2), 139—145
[16]	Liang F. Y., Wu R. R., Cao C. L., Zheng Y., Yang Z. H., Zhao F., Chem. J. Chinese Universities, 2014, 35(2), 372—376
	(梁方圆, 吴冉冉, 曹昌丽, 郑越, 杨朝晖, 赵峰. 高等学校化学学报, 2014, 35(2), 372—376)
[17]	Li J., Wang X. B., Yang J., Yang X. Y., Wan L., Chem. J. Chinese Universities, 2013, 34(4), 800—805
	(李静, 王贤保, 杨佳, 杨旭宇, 万丽. 高等学校化学学报, 2013, 34(4), 800—805)
[18]	Zhang J., Lan C., Shi B., Liu F., Zhao D., Tan X., Chem. Res. Chinese Universities, 2014, 30(6), 905—909
[19]	Faivre D., Böttger L. H., Matzanke B. F., Schüler D., Angew. Chem. Int. Ed., 2007, 46(44), 8495—8499
[20]	Staniland S., Ward B., Harrison A., van der Laan G., Telling N., Proce. Nat. Acad. Sci., 2007, 104(49), 19524—19528
[21]	Frankel R. B., Papaefthymiou G. C., Blakemore R. P., O'brien W., BBA-Molecul. Cell Res., 1983, 763(2), 147—159
[22]	Sun Y. C., Tao Y. G., Wei S. H., Cao N., Ge F., J. Anhui Polytech. Univ., 2012, 27(3), 13—16
	(孙忆晨, 陶玉贵, 魏胜华, 曹宁, 葛飞. 安徽工程大学学报, 2012, 27(3), 13—16)
[23]	Yamashita M., Rosatto S. S., Kubota L. T., J. Brazil Chem. Soc., 2002, 13(5), 635—641
[24]	Lexa D., Saveant J. M., Accounts Chem. Res., 1983, 16(7), 235—243
[25]	Okamoto A., Nakamura R., Hashimoto K., Electrochim. Acta, 2011, 56, 5526—5531

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