Construction of HRP Immobilized Enzyme Reactor Based on Hierarchically Porous Metal-organic Framework and Its Dye Degradation Application†

doi:10.7503/cjcu20200130

Abstract

Abstract:

Using the unstable Cu-MOF as template agent, the hierarchically porous Zr-MOF was prepared by a self-assembled template method. Horseradish peroxidase(HRP) was successfully immobilized into the hierarchically porous Zr-MOF via physical adsorption. The effect of pore size on catalytic activity of immobilized enzyme reactor was studied by changing the concentration of the pore size regulator benzoic acid(HBC). The effects of pH value of buffer solution, immobilized time and temperature on immobilized effect were investigated. The HRP-catalyzed degradation of crystal violet was used as a model reaction to study the operational stability and reusability of HRP@Zr-MOF. The results showed that the optimal conditions are the buffer pH=3.0, the immobilization time of 60 min and the temperature of 30 ℃, and the maximum immobilization was 61.6 mg/g. HRP@Zr-MOF showed better operational stability than the free enzyme. After 10 cycles, HRP@Zr-MOF can still remain 62.3% of the catalytic activity. When HRP@Zr-MOF was applied to the degradation of crystal violet in real water samples, they show very high catalytic efficiency with the degradation rate of more than 90% within 5 min.

Key words: Metal-organic framework(MOF), Horseradish peroxidase(HRP), Immobilized enzyme reactor, Dye degradation

CLC Number:

TrendMD:

GAO Xia,PAN Huibin,QIAO Chengfang,CHEN Fengying,ZHOU Yuan,YANG Wenhua. Construction of HRP Immobilized Enzyme Reactor Based on Hierarchically Porous Metal-organic Framework and Its Dye Degradation Application^†[J]. Chem. J. Chinese Universities, 2020, 41(7): 1591.

Figures/Tables 14

References 32

[1]	Lee C. K., Liu S. S., Juang L. C., Wang C. C., Lyu M. D., Hung S. H., J. Hazard. Mater., 2007, 148(3), 756—760 URL pmid: 17689860
[2]	An H. Q., Zhou J., Li J. X., Zhu B. L., Wang S. R., Zhang S. M., Wu S. H., Huang W. P., Catal. Commun., 2009, 11(3), 175—179
[3]	Ratanatawanate C., Tao Y., Balkus K. J ., J. Phys. Chem. C, 2009, 113(24), 10755—10760
[4]	Daoud M., Benturki O., Girods P., Donnot A., Fontana S., Microchem. J., 2019, 148, 493—502
[5]	Wang H. L., Zhao Y., Ma L. K., Fan P. H., Xu C. B., Jiao C. L., Lin A. J., Chem. J. Chinese Universities, 2016, 37(2), 335—341
	( 王会丽, 赵越, 马乐宽, 范鹏浩, 徐从斌, 焦春磊, 林爱军. 高等学校化学学报, 2016, 37(2), 335—341)
[6]	Yang J. X., Yan X. Y., Wang L., J. Shaanxi Normal University(Natural Science Edition), 2013, 41(5), 50—54
	( 杨菊香, 严向阳, 王旅. 陕西师范大学学报(自然科学版), 2013, 41(5), 50—54)
[7]	Valley B., Jing B., Ferreira M., Zhu Y. X ., ACS Appl. Mater. Interfaces, 2019, 117, 7472—7478
[8]	Solpan D., Guven O., Radiat. Phys. Chem., 2002, 65(4), 549—558
[9]	Zhou Q. X., Bull. Environ. Contam. Toxicol., 2001, 66(6), 784—793 URL pmid: 11353382
[10]	Sirirerkratana K., Kemacheevakul P., Chuangchote S., J. Cleaner Production, 2019, 215, 123—130
[11]	Kashefi S., Borghei S. M., Mahmoodi N. M., Microchem. J., 2019, 145, 547—558
[12]	Gao F. Q., Wang S., Wang Y. F., Zhao D. L., Cui R., Jiang Y. C., Chem. J. Chinese Universities, 2018, 39(5), 904—910
	( 高丰琴, 王珊, 王云芳, 赵丹蕾, 崔茹, 蒋育澄. 高等学校化学学报, 2018, 39(5), 904—910)
[13]	Bharagava R. N., Mani S., Mulla S. I., Saratale G. D., Ecotox. Environ. Safe., 2018, 156, 166—175
[14]	Kashefi S., Borghei S. M., Mahmoodi N. M., Microchem. J., 2019, 145, 547—558 doi: 10.1016/j.microc.2018.11.023 URL
[15]	Jiang W., Yang J. B., Wang X. H., Han H. B., Yang Y., Tang J., Li Q. S., Bioresource Technol., 2018, 247, 1246—1248
[16]	Ali M., Husain Q., Sultana S., Ahmad M., Chemosphere, 2018, 202, 198—207 URL pmid: 29571140
[17]	Tian N., Tian X. K., Nie Y. L., Yang C., Zhou Z. X., Li Y. Chem. Eng. J., 2018, 352, 469—476 doi: 10.1016/j.cej.2018.07.061 URL
[18]	Scott T., Zhao H., Deng W., Feng X., Li Y., Chemosphere, 2019, 216, 1—8 URL pmid: 30359911
[19]	Bilal M., Rasheed T., Zhao Y. P., Iqbal H. M. N., Int. J. Biol. Macromol., 2019, 124, 742—749 URL pmid: 30496859
[20]	Kim Y., Yang T., Yun G., Ghasemian M. B., Koo J., Lee E., Cho S. J., Kim K., Angew. Chem. Int. Ed., 2015, 54, 13273—13278
[21]	Pang S. L., Wu Y. W., Zhang X. Q., Li B. N., Ouyang J., Ding M. Y., Process Biochem., 2016, 51, 229—239
[22]	Cao Y., Wu Z. F., Wang T., Xiao Y., Huo Q. S., Liu Y. L., Dalton Trans., 2016, 45, 6998—7003 URL pmid: 26988724
[23]	Li P., Moon S. Y., Guelta M. A., Harvey S. P., Hupp J. T., Farha O. K., J. Am. Chem. Soc., 2016, 138, 8052—8055 URL pmid: 27341436
[24]	Wang Q. Q., Zhang X. P., Huang L., Zhang Z. Q., Dong S. J., Angew Chem. Int. Ed., 2017, 56(50), 16082—16085
[25]	Schejbal J., Glatz Z., J. Sep. Sci., 2018, 41(1), 323—335 doi: 10.1002/jssc.201700905 URL pmid: 28988452
[26]	Rafiei S., Tangestaninejad S., Horcajada P., Moghadam M., Mirkhani V., Mohammadpoor-Baltork I., Kardanpour R., Zadehahmadi F., Chem. Eng. J., 2018, 334, 1233—1241 doi: 10.1016/j.cej.2017.10.094 URL
[27]	Cui R., Bai C. H., Jiang Y. C., Hu M. C., Li S. N., Zhai Q. G., Chem. Eng. J., 2015, 259, 640—646 doi: 10.1016/j.cej.2014.08.074 URL
[28]	Roberge C., Amos D., Pollard D., Devine P., J. Mol. Catal. B: Enzym., 2009, 56, 41—45
[29]	Li X. X., Zhu H., Feng J., Zhang J. W., Deng X., Zhou B. F., Zhang H. L., Xue D. S., Li F. S., Mellors N. J., Li Y. F., Peng Y., Carbon, 2013, 60, 488—497
[30]	Yang Q., Wang B., Zhang Z., Lou D. S., Tan J., Zhu L. C., RSC Adv., 2017, 7, 38028—38036
[31]	Lykourinou V., Chen Y., Wang X., Meng L., Hoang T., Ming L., Musselman R. L., Ma S., J. Am. Chem. Soc., 2011, 133, 10382—10385 URL pmid: 21682253
[32]	Nadar S. S., Rathod V. K., Int. J. Biol. Macromol., 2017, 95, 511—519 URL pmid: 27889341

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Carrier	n(HBC)/mmol	Catalytic activity(%)	Hierarchically porous size/nm	Adsorption average pore diameter/nm
Zr-MOF-1	5	56.88	1.4/2.8/4.5	4.2
Zr-MOF-2	10	98.32	1.5/2.8/8.8	8.6
Zr-MOF-3	15	90.35	1.3/11.8/18.5	12.8

Carrier	n(HBC)/mmol	Catalytic activity(%)	Hierarchically porous size/nm	Adsorption average pore diameter/nm
Zr-MOF-1	5	56.88	1.4/2.8/4.5	4.2
Zr-MOF-2	10	98.32	1.5/2.8/8.8	8.6
Zr-MOF-3	15	90.35	1.3/11.8/18.5	12.8