PAPH2O2HRP Voltammetric Enzymelinked Immunoassay for Trace Thyroxine in Human Serum

Abstract

Abstract: A voltammetric enzymelinked immunoassay based on new system of p-aminophenol(PAP)H₂O₂-horseradish peroxidase(HRP) has firstly been developed and used for the detection of HRP and thyroxine(T₄). HRP or labelled HRP catalyzes the oxidation reaction of PAP with H₂O₂, the product of which produces a sensitive voltammetric peak at potential of -0.45 V(vs. SCE) in BrittonRobinson(B-R) buffer solution. By using this voltammetric peak, the detection limit to thyroxine(T₄)is 10 ng/mLand the linear range 1.0-320 ng/mL. The detection limit to T₄with this method is lower than that with the enzymelinked immunosorbent spectrophotometric assay(ELISA). Some human serum samples have been analyzed and satisfactory results have been obtained.

Key words: p-Aminophenol, Horseradish peroxidase(HRP), Immunoassay, Voltammetry, Thyroxine(T₄)

CLC Number:

O657.14

TrendMD:

ZHANG Shu-Sheng, JIAO Kui, CHEN Hong-Yuan . PAPH₂O₂HRP Voltammetric Enzymelinked Immunoassay for Trace Thyroxine in Human Serum[J]. Chem. J. Chinese Universities, 1999, 20(6): 881.

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PAPH₂O₂HRP Voltammetric Enzymelinked Immunoassay for Trace Thyroxine in Human Serum

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