Chem. J. Chinese Universities ›› 1997, Vol. 18 ›› Issue (12): 1963.

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Determination of Pyruvate in Human Serum Based on Chemiluminescent System of Ru (bipy)32+-Ce (Ⅳ)-pyruvate

LING Lian-Sheng, HE Zhi-Ke, CAI Ru-Xiu   

  1. Department of Chemistry, Wuhan University, Wuhan 430072
  • Received:1996-10-06 Online:1997-12-24 Published:1997-12-24

Abstract: The reaction of Ce (Ⅳ) with Ru (bipy)32+ (bipy=2, 2-bipyridine) and pyruvate produces chemiluminescence (CL) in aqueous solution.The effect of concentration of suIfate acid, Ce(Ⅳ) and Ru(bipy) on the CLintensity was studied.The results showed that CLintensity was linearly related to the concentration of pyruvate over the range of 4.0 ×10-8,8.10×10-6 mol/Lunder the optimum conditions.The method was applied successfully to determine pyruvate in normal human serum.

Key words: Chemiluminescence, Ru(bipy)32+, Pyruvate, Human serum

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