Interactions of Pyrene with Human Serum Albumin and Bovine Serum Albumin： Microenvironmental Polarity Differences at Binding Sites

doi:10.7503/cjcu20200559

Abstract

Abstract:

The interactions of pyrene（Pyr）， a microenvironmental hydrophobic fluorescent probe， with human serum albumin（HSA） or bovine serum albumin（BSA） were investigated using steady-state fluorescence， fluorescence resonance energy transfer technology， and molecular docking methods. It was observed that the average values of I₁/I₃ of the Pyr in the presence of HSA or BSA are 1.36 and 0.92， respectively. The binding constants of Pyr with HSA or BSA has been decreased from 1.86×10⁷ L/mol ^{to 1.71×10⁵ L/mol. The apparent distances of Pyr to tryptophan residues in HSA or BSA were calculated to be 2.37 and 2.34 nm. The binding sites of Pyr in HSA or BSA were located in subdomain ⅠB and subdomains ⅠA， respectively. The polarity of amino acid residues around the binding sites was one of the main factors affecting the I₁/I₃ value of Pyr. The experimental results suggested that there were significant differences in the binding sites and its microenvironmental polarity of BSA and HSA with Pyr.}

Key words: Pyrene, Human serum albumin, Bovine serum albumin, Microenvironmental polarity, Interaction mechanism

CLC Number:

O657

TrendMD:

LI Mengshuo, ZHANG Jing, LIU Dan, ZHU Yaxian, ZHANG Yong. Interactions of Pyrene with Human Serum Albumin and Bovine Serum Albumin： Microenvironmental Polarity Differences at Binding Sites[J]. Chem. J. Chinese Universities, 2021, 42(3): 731.

Figures/Tables 8

References 27

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System	K_sv/(L·mol^-1)	K_q/(mol^-1·s^-1)	r	T/K	pH	K_b/(L·mol^-1)	n	r
Pyr?HSA	3.80×10⁵	3.80×10¹³	0.96	298	7.40	1.86×10⁷	1.30	0.97
Pyr?BSA	2.33×10⁵	2.33×10¹³	0.96	298	7.40	1.71×10⁵	0.97	0.98

System	K_sv/(L·mol^-1)	K_q/(mol^-1·s^-1)	r	T/K	pH	K_b/(L·mol^-1)	n	r
Pyr?HSA	3.80×10⁵	3.80×10¹³	0.96	298	7.40	1.86×10⁷	1.30	0.97
Pyr?BSA	2.33×10⁵	2.33×10¹³	0.96	298	7.40	1.71×10⁵	0.97	0.98

System	R₀/nm	E(%)	r_DA/nm	K_ET/s^-1
Pyr?HSA	2.37	0.25	2.83	5.82×10⁷
Pyr?BSA	2.34	0.22	2.90	4.92×10⁷

System	R₀/nm	E(%)	r_DA/nm	K_ET/s^-1
Pyr?HSA	2.37	0.25	2.83	5.82×10⁷
Pyr?BSA	2.34	0.22	2.90	4.92×10⁷

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