Screening and Structures Characterization of Human Serum Albumin Ligands from Radix Aconiti Alkaloids Extract by Ultrafiltration LC-MSn

doi:10.7503/cjcu20120962

Chem. J. Chinese Universities ›› 2013, Vol. 34 ›› Issue (7): 1635.doi: 10.7503/cjcu20120962

• Analytical Chemistry • Previous Articles Next Articles

Screening and Structures Characterization of Human Serum Albumin Ligands from Radix Aconiti Alkaloids Extract by Ultrafiltration LC-MSⁿ

ZHU Hong-Bin^1,2, LIU Shu¹, WANG Chun-Yan^1,2, LIU Zhi-Qiang¹, SONG Feng-Rui¹

1. Changchun Centre of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China

Received:2012-10-25 Online:2013-07-10 Published:2013-06-21
Contact: SONG Feng-Rui E-mail:songfr@ciac.jl.cn

Abstract

Abstract:

A ultrafiltration LC-MS screening assay was developed for the screening and structures characterization of human serum albumin(HSA) ligands from the alkaloids extract of Radix aconiti. As a result, 9 compounds that can bind to HSA were found and were identified as 14-benzoylmesaconine, 14-benzoylaconine, 10-OH-mesaconitine, mesaconitine, 10-OH-aconitine, aconitine, hypaconitine, deoxyaconitine and 3,13-deoxyaconitine by ESI-MSⁿ data.

Key words: Radix aconiti, Ultrafiltration LC-MS, Human serum albumin

CLC Number:

O657.63

TrendMD:

ZHU Hong-Bin, LIU Shu, WANG Chun-Yan, LIU Zhi-Qiang, SONG Feng-Rui. Screening and Structures Characterization of Human Serum Albumin Ligands from Radix Aconiti Alkaloids Extract by Ultrafiltration LC-MSⁿ[J]. Chem. J. Chinese Universities, 2013, 34(7): 1635.

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Screening and Structures Characterization of Human Serum Albumin Ligands from Radix Aconiti Alkaloids Extract by Ultrafiltration LC-MSⁿ

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