Matrix-assisted Laser Desorption Ionization Mass Spectrometry for Determination of Molecular Weight of CaMs

Chem. J. Chinese Universities ›› 1997, Vol. 18 ›› Issue (12): 1966.

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Matrix-assisted Laser Desorption Ionization Mass Spectrometry for Determination of Molecular Weight of CaMs

LlU De-Long¹, ZHU Zhi-Hua², ZHAO Shan-Kai², SUN Da-Ye³, YANG Yan-Sheng¹

1. Department of Chemistry, Analytical and Testing Centert, Zhongshan University, Guangzhou 510275;
2. Analytical and Testing Centert, Zhongshan University, Guangzhou 510275;
3. Department of Biology, Hebei Normal University, Shijiazhuang 050016

Received:1996-11-28 Online:1997-12-24 Published:1997-12-24

Abstract

Abstract: The molecular weight of bovine brain CaM, intracellular CaMand extracellularCaMof cauliflower have been determined for the first time by Matrix-Assisted Larer Desorp tion Ionization Time of Flight Mass Spectrometry(MALDI-TOF-MS).The molecular weightof bovine brain CaM, 16699.9 Da determined by MALDI-TOF-MS, is closely ideatical with16700 Da, calculated from its primary sequence, showing the high accuracy of MALDITOF-MSand the conf1rmation of previously determined sequence.Furthermore, the molecular weight of intracellular CaMand extracellular CaMdetermined by MAlDI-TOF-MS, 17025 Da and 15582 Da, are in agreement with 17500 Da and 15000 Da determined by SDSPAGE-This agreement shows the high accuracy of MALDI-TOF-MS.In addition, themolecular weight of CaMs with this technique can be determined rapidly(less than 10 min)and sensitively.

Key words: MALDI-TOF-MS, Calmodulin, Extracellular calmodulin, Determination of molecular weight

CLC Number:

O657.6

TrendMD:

LlU De-Long, ZHU Zhi-Hua, ZHAO Shan-Kai, SUN Da-Ye, YANG Yan-Sheng . Matrix-assisted Laser Desorption Ionization Mass Spectrometry for Determination of Molecular Weight of CaMs[J]. Chem. J. Chinese Universities, 1997, 18(12): 1966.

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Matrix-assisted Laser Desorption Ionization Mass Spectrometry for Determination of Molecular Weight of CaMs

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