Piezoelectric Immunosensor Based on the Agglutination of PEG for Determination of S. Japonicum Antibody

Abstract

Abstract: Anew liquid piezoelectric immunoassay was developed to determine infectionrabbit serum (IRS) by adult worm antigen (AWA) of S.japonicum, which is based on thetination of antibody and antigen-PEGcomplex by an immunoreaction.By examining thesurface appearances of the quartz crystal with scanning electronic microscopy(SEM) technologyand investigating the effect of the density and viscousity of the solution, it can be found thatthe absorption on the surfaces of the quartz crystal plays a dominant role in the frequencyresponse in the immunoassay.The influences including PEGconcentration, complexing time ofAWA-PEGcomplex and detection temperature on frequency responses were investigated indetail, respectively.The frequency shift was linear with the antibody concentration in therange of 3.6-42.0μg/mL.Five rabbit serum specimens were analyzed with this method andthe analytical results given by this approach show that the detection ratio of positive serumreaches 100% and the antibody concentration can be quantitatively determined.

Key words: PEG, Liquid piezoelectric immunoassay, S. japonicum antibody

CLC Number:

O657

TrendMD:

WU Zhao-Yang, SHEN Guo-Li, YU Ru-Qin, WANG Shi-Ping, ZENG Xian-Fang. Piezoelectric Immunosensor Based on the Agglutination of PEG for Determination of S. Japonicum Antibody[J]. Chem. J. Chinese Universities, 1997, 18(11): 1774.

References

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