Preparation and Properties of Double Network Hydrogels Based on Gellan Gum and Polyethylene Glycol Acrylate†

doi:10.7503/cjcu20160650

Abstract

Abstract:

Hydrogels have the similar 3D micro-environment as the native extracellular matrix and hold great potential in future biomedical applications. However the mechanical performance limited their applications. The double network hydrogel(DN gel) attracts much attention due to its high mechanical properties. This study aimed to prepare a series of DN gels based on gellan gum and polyethylene glycol acrylate(PEGDA) to improve the toughness of gellan gum hydrogel for 3D cell culture and tissue repair. The DN hydrogels exhibited better mechanical properties, with the tensile fracture energy up to 1.01 × 10³ J/m², which was similar to the value of natural articular cartilage. Our study suggests that the DN gel based on gellan gum and PEGDA present promising mechanical performance and cell seeding efficiency. These hydrogels may have prospective applications in the fields of wound dressing, artificial cartilage and tissue engineering scaffold materials which require high mechanical properties.

Key words: Gellan gum, Double network hydrogel, Polyethylene glycol acrylate(PEGDA), 3D cell culture

CLC Number:

O631
TB324

TrendMD:

WANG Zhengguang, HU Duo, WU Dongwei, LU Lu, ZHOU Changren. Preparation and Properties of Double Network Hydrogels Based on Gellan Gum and Polyethylene Glycol Acrylate^†[J]. Chem. J. Chinese Universities, 2017, 38(2): 275.

Figures/Tables 12

Fig.1 Chemical structures of native gellan gum(A) and deacylated gellan gum(B) repeat unit

Scheme 1 Synthesis of poly(ethylene glycol) diacrylate(PEGDA)

Fig.2 FTIR-ATR spectra of PEG(a) and PEGDA(b)

Fig.3 1H NMR spectra of PEG(a) and PEGDA(b)

Fig.4 Angular frequency dependence of storage modulus G'(solid)and loss modulus G″(hollow) for single- and double-network hydrogels(A) and double-network hydrogel with different UV irradiation time(B)

Fig.5 Tensile properties of the hydrogels(A) GG; (B) PEGDA; (C) I-2959; (D) Na+.

Fig.6 Dynamic mechanical analysis of the hydrogelsInset: Dynamic compression of the first five cycles.

Fig.7 Equilibrium swelling curves of the hydrogels GG(a), PEGDA(b) and PG(c) in PBS solution

Fig.8 SEM images of the hydrogels(A) and (B) 0.5%GG; (C) 15%PEGDA; (D) 0.5%GG-15%PEGDA DN hydrogel.

Fig.9 Pore size and distribution of the hydrogels GG(A), PEGDA(B) and PG(C)

Scheme 2 Structure of GG/PEGDA DN hydrogel

Fig.10 Live/dead staining of the encapsulated cells in the hydrogels GG(A1—C1) and PG(A2—C2) indicates high cell viability after cultured for 3 d(A1, A2) and 5 d(B1, B2) and 7 d(C1, C2)Green: live cells; red: dead cells.

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