Synthesis, Characterization and Micellization of Star-shaped Amphiphilic Block Copolymer 6-s-PLGAx-PEG-NH2

doi:10.7503/cjcu20120730

Chem. J. Chinese Universities ›› 2013, Vol. 34 ›› Issue (4): 1014.doi: 10.7503/cjcu20120730

• Polymer Chemistry • Previous Articles Next Articles

Synthesis, Characterization and Micellization of Star-shaped Amphiphilic Block Copolymer 6-s-PLGA_x-PEG-NH₂

CHEN Yong-Xia¹, YANG Zi-Ying¹, WANG Liang³, ZHANG Zheng-Pu², KONG De-Ling¹, YANG Jing¹, SUN Hong-Fan¹, SONG Cun-Xian¹

1. Tianjin Key Laboratory of Biomaterial Research, Institute of Biomedical Engineering, Peking Union Medical College, Chinese Academy of Medical Science, Tianjin 300192, China;
2. Institute of Polymer Chemistry, Nankai University, Tianjin 300071, China;
3. Chemistry and Chemical Engineering, Tianjin University of Technology, Tianjin 300384, China

Received:2012-07-30 Online:2013-04-10 Published:2013-03-15

Abstract

Abstract:

The star-shaped poly(lactide-co-glycolide)(PLGA) was synthesized via the ring-opening polymerization of D,L-lactide and glycolide, with inositol as a multifunctional initiator and stannous 2-ethylhexanoate as a catalyst. Amine-terminated poly(ethylene glycol)(NH₂-PEG-NH₂) was synthesized using improved Gabriel method, in which the end hydroxyl groups were converted to amino groups. A series of 6-s-PLGA_x-PEG-NH₂ was prepared using classical DCC condensation reaction in the mild condition. The structures of these polymers were characterized by ¹H NMR spectroscopy, while the molecular weight and polydispersity index(PDI) were determined by gel permeation chromatography(GPC). The glass transition temperature(T_g) of copolymer was determined by differential scanning calorimetry(DSC). The NH₂-PEG-NH₂ was characterized using IR and element analyses, the result of element analysis proved that the hydroxyl conversion was up to 90%. Nanoparticles(NPs) were prepared by solvent evaporation method, ¹H NMR was used to analysis the structure of the NPs, dynamic light scatering(DLS) for the size and size distribution, transition electron microscope(TEM) for the morphology of the NPs. The biocompatibility was detected with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltertrazolium bromide(MTT) assay. The results showed success of synthesis of star-shaped amphiphilic block copolymer 6-PLGA_x-PEG-NH₂. The new polymer has good biocompatibility and can formulate micelles easily. It may be used as a new drug carrier for drug controlled system.

Key words: Star-shaped poly(lactide-co-glycolide), Nano-micelle, Amphiphilic star-shaped PLGA-PEG-NH₂

CLC Number:

O632

TrendMD:

CHEN Yong-Xia, YANG Zi-Ying, WANG Liang, ZHANG Zheng-Pu, KONG De-Ling, YANG Jing, SUN Hong-Fan, SONG Cun-Xian. Synthesis, Characterization and Micellization of Star-shaped Amphiphilic Block Copolymer 6-s-PLGA_x-PEG-NH₂[J]. Chem. J. Chinese Universities, 2013, 34(4): 1014.

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Synthesis, Characterization and Micellization of Star-shaped Amphiphilic Block Copolymer 6-s-PLGA_x-PEG-NH₂

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