Preparation and the Antibacterial Activity of AgNPs/PLGA-PEG-PLGA Composite Hydrogel †

doi:10.7503/cjcu20190351

Abstract

Abstract:

The PLGA-PEG-PLGA triblock thermosensitive hydrogel was synthesized via ring opening copolymerization of D,L-lactide(D,L-LA) and glycolide(GA) with PEG1000 as a macromolecular initiator. ¹H NMR spectrum of the copolymer was obtained to confirm the structure and composition. Silver nanoparticles which prepared by reduction of silver nitrate were mixed with the prepared PLGA-PEG-PLGA three block copolymer hydrogel. A new type of AgNPs/PLGA-PEG-PLGA hydrogel was prepared. The properties of AgNPs/PLGA-PEG-PLGA composite hydrogels were characterized. The AgNPs/PLGA-PEG-PLGA composite hydrogel still has a temperature sensitive property, and the sol-gel phase transition occurs with the increase of temperature. Novel hydrogel can be sustained release of silver nanoparticles to play antibacterial properties. In vitro biocompatibility experiments showed that the novel AgNPs/PLGA-PEG-PLGA hydrogel has good biocompatibility and no cytotoxicity. It is a new type of composite hydrogel with good application prospect.

Key words: PLGA-PEG-PLGA, Thermosensitive hydrogel, AgNPs, Antibacterial

CLC Number:

O632

TrendMD:

YUAN Baoming,DONG Xiaoming,YANG Fan,PENG Chuangang,WANG Jincheng,Wu Dankai. Preparation and the Antibacterial Activity of AgNPs/PLGA-PEG-PLGA Composite Hydrogel ^†[J]. Chem. J. Chinese Universities, 2019, 40(10): 2225.

Figures/Tables 10

Fig.1 1H NMR spectrum of PLGA-PEG-PLGA in CDCl3

Fig.2 Temperature-dependent sol-gel phase transitions of the copolymer solutions Insets: (Ⅰ) sol, <35 ℃; (Ⅱ) gel, 35—65 ℃; (Ⅲ) precipition, >65 ℃.

Fig.3 In vitro mass loss profiles for the PLGA-PEG-PLGA hydrogels with polymer concentration of 25%

Fig.4 UV-Vis spectrum of AgNPs

Fig.5 XRD patterns of hydrogel(a), AgNPs(b) and AgNPs/hydrogel(c)

Fig.6 TEM images of AgNPs(A), PLGA-PEG-PLGA(B) and Ag-NPs/PLGA-PEG-PLGA(C) The arrow shows AgNPs encapsulated in PLGA-PEG-PLGA micelles.

Fig.7 Sol(A) and gel(B) phases of AgNPs/PLGA-PEG-PLGA

Fig.8 Storage moduli of the hydrogels and AgNPs/PLGA-PEG-PLGA a. 25%Gel; b. 0.0125%Ag/25%gel; c. 0.025%Ag/25%gel.

Fig.9 In vitro cytotoxicities of PLGA-PEG-PLGA gel and Ag/PLGA-PEG-PLGA composite hydrogel extracts with different AgNPs concentrations to L929 cells with PEI 25K as a positive control Data are presented as mean±standard deviation(n=3). a. 25%Gel; b. 0.00625%Ag; c. 0.0125%Ag; d. 0.025%Ag; e. 0.05%Ag; f. 0.1%Ag; g. Tris.

Fig.10 Antibacterial ring experiment of AgNPs/PLGA-PEG-PLGA composite hydrogel

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