Chem. J. Chinese Universities ›› 1997, Vol. 18 ›› Issue (11): 1769.
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CHEN Hong, SONG Li-Guo, XIONG Shao-Xiang, CHENG Jie-Ke
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Abstract: We demonstrate that hydroxyethyl cellulose, sucrose and boric acid can formnongel sieving matrix with interpenetrating polymer networks (INPs).This kind of INPs mixednon-gel sieving matrix can greatly improve DNAseparation efficiency.While all elevenfragments of φX174/Hae Ⅲ DNAcan not be completely separated with a sieving matrix of 0.8%hydroxyethyl cellulose in 1×TBE buffer, they are successfully separated by a non-gelsieving matrix of 0.8% hydroxyethyl cellulose, 10% sucrose in 1×TBEbuffer.The reactionmechnism to form the INPs has been proposed.The length of a PCRmagnified DNAfragment from sex-related gene in rice-field cells is also determined, which is in good agreementwith slab gel electrophoresis.
Key words: Non-gel sieving capillary electrophoresis, DNA fragment, Laser-induced fluorescence, Charge-coupled device, PCR amplified product
CLC Number:
O657.8
TrendMD:
CHEN Hong, SONG Li-Guo, XIONG Shao-Xiang, CHENG Jie-Ke. Separation of DNA Fragments by Capillary Electrophoresis with Non-gel Sieving Matrix of Hydroxyethyl Cellulose and Sucrose[J]. Chem. J. Chinese Universities, 1997, 18(11): 1769.
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http://www.cjcu.jlu.edu.cn/EN/Y1997/V18/I11/1769