高等学校化学学报

高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (2): 267.

• 研究简报 • 上一篇    下一篇

应用停流时间分辨光谱研究氧合血红蛋白与过氧化氢反应中间体的动力学和机理

王瑞勇, 蔡汝秀, 林智信   

  1. 武汉大学化学与分子科学学院, 武汉 430072
  • 收稿日期:2003-02-21 出版日期:2004-02-24 发布日期:2004-02-24
  • 通讯作者: 蔡汝秀(1939年出生),女,教授,博士生导师,从事动态分子光谱分析及动力学研究.E-mail;cai-lin@whu.edu.cn E-mail:cai-lin@whu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号;20275027)资助

Studies on the Kinetics and Mechanism of Intermediates of Rexaction between Oxyhemoglobin and H2O2 Using Stopped-flow Time-resolved Spectrophotometry

WANG Rui-Yong, CAI Ru-Xiu, LIN Zhi-Xin   

  1. Department of Chemistry, Wuhan University, Wuhan 430072, China
  • Received:2003-02-21 Online:2004-02-24 Published:2004-02-24

PDF (PC)

被引次数

3

关键词: 停流时间分辨光谱, 氧合血红蛋白, 过氧化氢, 机理

Abstract: Interaction between oxyhemoglobin and H2O2 was studied by the aid of stopped-flow time-resolved spectrophotometry. The consecutive reaction kinetics of oxyhemoglobin with H2O2 suggested the presence of an intermediate species. The influence of pH, concentration of H2O2, and temperature on the pseudo-first order rate constants was studied. The kinetic experiments performed at different temperatures allowed to calculate the activation energy. A reasonable mechanism was also proposed.

Key words: Stopped-flow time-resolved spectrophotometry, Oxyhemoglobin, Hydrogen peroxide, Mechanism

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