高等学校化学学报

高等学校化学学报 ›› 2003, Vol. 24 ›› Issue (10): 1787.

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中性红荧光探针法测定生物大分子核酸

李文友1, 吴会灵1, 何锡文1, 梁宏2   

  1. 1. 南开大学化学系, 元素有机化学国家重点实验室, 天津 300071;
    2. 广西师范大学化学系, 桂林 541004
  • 收稿日期:2002-03-20 出版日期:2003-10-24 发布日期:2003-10-24
  • 通讯作者: 何锡文(1939年出生),男,教授,博士生导师,从事分子模板及分子识别研究.
  • 基金资助:

    国家自然科学基金(批准号:20175009);教育部博士学科点专项基金(批准号:20020055002);广西省教育厅研究项目资助

Determination of Trace Nucleic Acids Using Neutral Red as the Fluorescent Probe

LI Wen-You1, WU Hui-Ling1, HE Xi-Wen1, LIANG Hong2   

  1. 1. Department of Chemistry, State Key Laboratory of Elemento-organic Chemistry, Nankai University, Tianjin 300071, China;
    2. Department of Chemistry, Guangxi NormaLUniversity, Guilin 541004, China
  • Received:2002-03-20 Online:2003-10-24 Published:2003-10-24

PDF (PC)

被引次数

45

关键词: 中性红, 核酸, 荧光分析法

Abstract: The fluorescence spectra and binding reaction of neutral red with nucleic acids have been studied.At pH=4.5, a new method for the determination of trace nucleic acids based on fluorescence quenching measurement was established.When 8.0×10-6mol/L NRis employed, the linear ranges of the calibration graphs are 0.08-2.0 μg/mLfor calf thymus DNA(ctDNA), 0.12-2.2 μg/mLfor fish sperm DNA(fsDNA) and 0.30-1.4 μg/mLfor yeast RNA(yRNA), respectively.The corresponding detection limits are 30, 33 and 66ng/mL, respectively.The real samples were analyzed satisfactorily.

Key words: Neutral red, Nucleic acids, Fluorescence analysis

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