高等学校化学学报 ›› 2021, Vol. 42 ›› Issue (11): 3284.doi: 10.7503/cjcu20210451

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基于DNA纳米结构的细胞间相互作用的调控

胡灵, 殷垚, 柯国梁(), 张晓兵   

  1. 湖南大学化学化工学院, 化学生物传感与计量学国家重点实验室, 长沙 410082
  • 收稿日期:2021-06-30 出版日期:2021-11-10 发布日期:2021-08-20
  • 通讯作者: 柯国梁 E-mail:glke@hnu.edu.cn
  • 基金资助:
    国家自然科学基金(21890744)

Regulation of Cell-cell Interactions Based on DNA Nanostructures

HU Ling, YIN Yao, KE Guoliang(), ZHANG Xiaobing   

  1. State Key Laboratory of Chemo/Biosensing and Chemometrics,College of Chemistry and Chemical Engineering,Hunan University,Changsha 410082,China
  • Received:2021-06-30 Online:2021-11-10 Published:2021-08-20
  • Contact: KE Guoliang E-mail:glke@hnu.edu.cn
  • Supported by:
    the National Natural Science Foundation of China(21890744)

摘要:

细胞通过化学信号、 电子交换和直接接触等方式交换彼此之间的物质和信息, 以调节生命体的生长发育. 因此, 细胞间的相互作用研究与调控在细胞功能的机制研究和疾病的诊断及治疗等领域具有非常重要的意义. DNA纳米结构具有易合成、 易修饰、 可编程性设计及生物安全性高等优点, 有望实现操作简单、 精确可调、 智能响应的细胞间相互作用调控, 受到了广泛关注. 本文综述了寡核苷酸链杂交、 受体-配体结合和核酸适体靶向识别等基于DNA纳米结构的细胞组装策略, 总结了pH调控、 金属离子调控和DNA链激活等细胞间相互作用的调控手段, 并重点介绍了其在细胞间作用力的测量和成像、 体外组织模型的构建、 细胞间的通讯交流和细胞免疫治疗等领域的应用. 最后对该领域进行了总结和展望, 希望为相关研究提供有益参考.

关键词: 脱氧核糖核酸纳米结构, 细胞间相互作用, 细胞组装, 调控手段

Abstract:

Cells exchange materials and information with each other through chemical signals, electronic exchange and direct contact, to regulate the growth and development of living organisms. Therefore, the research and regulation of cell-cell interaction are great significance in mechanism study of cell functions and the diagnosis and treatment of diseases. DNA nanostructures have the advantages of easy synthesis, easy modifi- cation, programmable design, and high biological safety, which are promising for cell-cell interactions research with simple operation, precise adjustment, and intelligent response. In this review, we summarized the DNA nanostructure-based cell assembly strategies such as oligonucleotide hybridization, receptor-ligand binding, and aptamer targeted recognition, then introduced some regulation means such as pH, metal ions, and DNA triggers. The discussion also focused on some applications of cell-cell interactions, including intercellular force measurement and imaging, in vitro tissue construction, cell-cell communication and cellular immunotherapy. Finally, the opportunity and challenge in this field were discussed.

Key words: DNA nanostructures, Cell-cell interaction, Cell assembly, Regulatory method

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