高等学校化学学报

高等学校化学学报 ›› 2001, Vol. 22 ›› Issue (7): 1077.

• 研究论文 •    下一篇

具有不同电荷和手性的Co(Ⅲ)配合物跨人红细胞膜的动力学研究

吴刚, 李荣昌, 乔秀文, 王坚成   

  1. 北京大学药学院化学生物系, 北京 100083
  • 收稿日期:2000-08-15 出版日期:2001-07-24 发布日期:2001-07-24
  • 通讯作者: 李荣昌(1941年出生),男,教授,从事生物无机化学研究.
  • 基金资助:

    国家自然科学基金(批准号:29571007)

Studies on the Kinetics of Cobalt(Ⅲ) Complexes with Various Charge and Chirality Crossing Human Erythrocyte Membrane

WU Gang, LI Rong-Chang, QIAO Xiu-Wen, WANG Jian-Cheng   

  1. Department of Chemical Biology, Pharmaceutical Science School of Peking University, Beijing 100083, China
  • Received:2000-08-15 Online:2001-07-24 Published:2001-07-24

PDF (PC)

被引次数

25

摘要： 研究了4种不同电荷的Co(Ⅲ)金属配合物跨人红细胞膜的动力学,并测定了它们跨人红细胞膜的一级反应动力学速率常数,发现[Co(C2O4)3]3-的跨膜速率明显高于[Co(en)3]3+,[Co(en)2(C2O4)]+和[Co(en)(C2O4)2]-,后3种配合物的跨膜速率常数随正电荷的减少略有增加,跨膜机制为简单扩散.[Co(C2O4)3]3-的跨膜速率受阴离子通道抑制剂DIDS明显抑制,抑制率为51.95%,推测其跨膜机制为部分经阴离子通道协同简单扩散过膜.人红细胞摄入L-[Co(C2O4)3]3-的速率明显大于D-[Co(C2O4)3]3-,显示了一定的手性选择性.

关键词: 钴, 人红细胞, 动力学

Abstract: The kinetics that four complexes with various charge and chirality crossed human erythrocyte membrane was studied, and their rate constants(k) of first-order kinetics were determined. The experimental results showed that the [Co(C2O4)3]3- has an obviously higher rate of cross-membrane[Co(en)3]3+than, [Co(en)2(C2O4)]+ and [Co(en)(C2O4)2]-,[Co(en)2(C2O4)]+and[Co(en)(C2O4)2]- the k values of the latter three kinds of complexes increased gradually with the decreasing of their positive charge, their mechanism of transmembrane transport are simple passive diffusion. The rate of transmembrane transport of [Co(C2O4)3]3- was obviously inhibited by DIDS, an anion channel inhibitor, the inhibition rate is 51.95%, its mechanism of transmembrane transport has been considered involving anion channel transport and passive diffusion. The uptake rate of L-[Co(C2O4)3]3- is larger than that of D-[Co(C2O4)3]3- by human erythrocytes, this exhibited that there was chiral selectivity when erythrocytes took up the optical isomers.

Key words: Cobalt, Human erythrocytes, Kinetics

中图分类号: 

TrendMD: