高等学校化学学报

高等学校化学学报 ›› 2001, Vol. 22 ›› Issue (5): 771.

• 研究论文 • 上一篇    下一篇

羟基磷灰石对牛血清白蛋白的吸附特性研究

殷钢, 詹劲, 刘铮, 袁乃驹   

  1. 清华大学化工系, 北京 100084
  • 收稿日期:2000-03-16 出版日期:2001-05-24 发布日期:2001-05-24
  • 通讯作者: 刘　铮(1964年出生),男,博士,教授,博士生导师,从事新型生物分离技术与设备的研究.Email:liuzheng@mail.tsinghua.edu.cn E-mail:liuzheng@mail.tsinghua.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:29676022);北京市自然科学基金(批准号:2992016)资助

Characterization of the Adsorption of Bovine Serum Albumin on Hydroxylapatite

YIN Gang, ZHAN Jin, LIU Zheng, YUAN Nai-Ju   

  1. Department of Chemical Engineering, Tsinghua University, Beijing 100084, China
  • Received:2000-03-16 Online:2001-05-24 Published:2001-05-24

PDF (PC)

被引次数

111

摘要： 研究了羟基磷灰石表面电位随溶液PO43-浓度、Ca2+浓度、离子强度和pH的变化规律;测定了不同操作条件下牛血清白蛋白在羟基磷灰石上的吸附容量;吸附等温线的测定结果表明该吸附属于Langmuir型;通过对该吸附过程的动力学研究,计算得到的表观活化能和吸附热数值较低,表明该吸附是物理吸附.

关键词: 羟基磷灰石, 牛血清白蛋白, Zeta电位, 吸附, 动力学

Abstract: Experimental studies on the effects of the concentration of PO43- and Ca 2+, ion strength and pHof buffer on the polarity and magnitude of F-potential of hydroxylapatite(HAP) were carried out in present study to characterize the surface physicochemical properties of hydroxylapatite. Adsorption of bovine serum albumin(BSA) on HAPwas carried at different conditions in terms of pH, ionic strength, and the concentration of PO43- to establish the knowledge of adsorption mechanism. A Langmuir adsorption isotherm was obtained and the apparent activation energy and adsorption heat were interpreted. The low magnitude of the apparent activation energy and the adsorption heat indicated the fast adsorption of BSAon hydroxylapatite was a physical adsorption in nature.

Key words: Hydroxylapatite, Bovine serum albumin, Zeta potential, Adsorption, Kinetics

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