高等学校化学学报

高等学校化学学报 ›› 1998, Vol. 19 ›› Issue (6): 876.

• 研究简报 • 上一篇    下一篇

Tb(3+)与氧合血红蛋白反应动力学的停流荧光光度法研究

程驿1, 林华宽2, 王夔3   

  1. 1. 南京大学化学系, 南京, 210093;
    2. 南开大学化学系, 天津, 300071;
    3. 北京医科大学药学院, 北京, 100083
  • 收稿日期:1997-05-16 出版日期:1998-06-24 发布日期:1998-06-24
  • 通讯作者: 王夔
  • 作者简介:程驿,男,31岁,博±研究生.
  • 基金资助:

    国家自然科学基金

Studies on Kinetics of Reaction Between Tb 3+ and Human Oxyhemoglobin by Stopped-flow Fluorometry

CHENG Yi1, LIN Hua-Kuan2, WANG Kui3   

  1. 1. Department of Chemistry, Nanjing University, Nanjing, 210093;
    2. Department of Chemistry, Nankai University, Tianjin, 300071;
    3. Department of Bioinorganic Chemistry, Beijing Medical University, Beijing, 100083
  • Received:1997-05-16 Online:1998-06-24 Published:1998-06-24

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关键词: 稀土, 氧合血红蛋白, 动力学, 停流荧光法

Abstract: The kinetics of reaction between Tb 3+ and human oxyhemoglobin was investigated by means of stopped flow fluorometry. The Tb 3+ ions quenched the intrinsic fluorescence of oxyhemoglobin within the first 100 ms. The rate constant of the reaction at various temperature and activation parameters were obtained. The results indicate that Tb 3+ binding to oxyhemoglobin is attributed to the second order reaction. The mechanism of the reaction was explored.

Key words: Lanthanide, Oxyhemoglobin, Kinetics, Stopped-flow fluorometry

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