高等学校化学学报

高等学校化学学报 ›› 1995, Vol. 16 ›› Issue (11): 1700.

• 研究简报 • 上一篇    下一篇

Cu(Ⅱ)对牛血SOD活性影响的研究

徐通敏, 周天舒, 金利通   

  1. 华东师范大学化学系, 上海, 200062
  • 收稿日期:1994-12-13 修回日期:1995-03-19 出版日期:1995-11-24 发布日期:1995-11-24
  • 通讯作者: 金利通
  • 作者简介:徐通敏,男,57岁,副教授.

The Influence of Cu(Ⅱ)on Bovine Superoxide Dismustase's Activity

XU Tong-Min, ZHOU Tian-Shu, JIN Li-Tong   

  1. Department of Chemistry, East China Normal University, Shanghai, 200062
  • Received:1994-12-13 Revised:1995-03-19 Online:1995-11-24 Published:1995-11-24

PDF (PC)

被引次数

11

关键词: 超氧化物歧化酶(SOD), 铜, 酶活性

Abstract: The relation between the content of the cofactor Cu2+of bovine superoxide dismustase and enzyme's activity is studied.Different amount of Cu2+is added into SOD solution,then Cu2+in SOD reaches new distribution balance.The content of free Cu2+is determined by GFAAS after preconcentration on Nafion modified tungsten coil,and the enzyme's activity is detected by the method of 1,2,3-trihydroxybenzene auto-oxidation.

Key words: Superoxide dismutase, Copper, Enzyme's activity

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