Chem. J. Chinese Universities ›› 2014, Vol. 35 ›› Issue (1): 30.doi: 10.7503/cjcu20130854
• Analytical Chemistry • Previous Articles Next Articles
XIE Fei, ZHANG Ying, JIN Wei, PAN Tingting, ZHOU Chao, REN Hao, JIN Qinhan, MU Ying*(
)
Received:2013-09-02
Online:2014-01-10
Published:2013-12-05
Contact:
MU Ying
E-mail:muying@zju.edu.cn
Supported by:CLC Number:
TrendMD:
XIE Fei, ZHANG Ying, JIN Wei, PAN Tingting, ZHOU Chao, REN Hao, JIN Qinhan, MU Ying. Detection of Inhibitory Effects of Suramin and Cisplatin on Hepatocellular Carcinoma Cells Using Surface Plasmon Resonance Imaging†[J]. Chem. J. Chinese Universities, 2014, 35(1): 30.
Fig.1 Schematic diagram(A) and physical map(B) of the SPRi system
Fig.2 Schematic diagrams of the two different types of PDMS microfluidic chips without micro-posts(A) and with micro-posts(B)
Fig.3 Production process of the flow cell
Fig.4 Physical map(A), host computer interface(B) and surface homogeneity(C) of the microfluidic chipDifferent areas of the sensing surface showed approximately the same signal intensity when 0.1 mol/L anti-CEA antibody solution flowed on the sensing surface.
Fig.5 SPRi response signals of the two different types of microfluidic chipsa. Without micro posts; b. with micro posts.
Fig.6 Function simulations of both flow cells without micro-posts(A) and with an array of micro-posts(B)
Fig.7 Differential SPRi sensorgrams against various concentrations of anti-cancer drugs after 24 h (A) Cisplatin treatment for 24 h; (B) suramin treatment for 24 h.
Fig.8 Differential SPRi sensorgrams against various concentrations of anti-cancer drugs after 48 h(A) Cisplatin treatment for 48 h; (B) suramin treatment for 48 h.
Fig.9 Inhibitory effects of suramin and cisplatin on the proliferation of HepG2 cells(A) At 24 h after the addition of cisplatin; (B) at 24 h after the addition of suramin.The results are shown as the mean±the standard of 3 independent experiments.
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